Âne anti-Chévre IgG Anticorps (DyLight 488) - Preadsorbed

N° du produit ABIN6698858

N° du produit (Fournisseur): 605-741-125

Aperçu rapide pour Âne anti-Chévre IgG Anticorps (DyLight 488) - Preadsorbed (ABIN6698858)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: DyLight 488

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Détail du produit

Reactivité: Chévre

Hôte: Âne

N° du produit (Fournisseur): 605-741-125

Fournisseur: Rockland

Fonction: Goat IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins

Attributs du produit: donkey anti-Goat IgG Antibody DyLight™ 488 Conjugation, donkey anti-Goat IgG DyLight™ 488 Conjugated Antibody,Anti-Goat IgG DyLight Antibody generated in donkey detects goat IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogène: Goat IgG whole molecule

Labeling Ratio: 5.6

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Commentaires:

Anti-Goat IgG DyLight 488 Antibody has been tested by dot blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, Multiplex

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Onoda, Hagiwara, Kubota, Yanagita, Takeda, Umezawa: "A Novel Staining Method for Detection of Brain Perivascular Injuries Induced by Nanoparticle: Periodic Acid-Schiff and Immunohistochemical Double-Staining." dans: Frontiers in toxicology, Vol. 4, pp. 825984, (2022) (PubMed).

Iguchi, Carrasco, Xie, Pineda, Malykhina, Wilcox: "Functional constipation induces bladder overactivity associated with upregulations of Htr2 and Trpv2 pathways." dans: Scientific reports, Vol. 11, Issue 1, pp. 1149, (2021) (PubMed).

Onoda, Kawasaki, Tsukiyama, Takeda, Umezawa: "Carbon nanoparticles induce endoplasmic reticulum stress around blood vessels with accumulation of misfolded proteins in the developing brain of offspring." dans: Scientific reports, Vol. 10, Issue 1, pp. 10028, (2020) (PubMed).

Umezawa, Onoda, Korshunova, Jensen, Koponen, Jensen, Khodosevich, Vogel, Hougaard: "Maternal inhalation of carbon black nanoparticles induces neurodevelopmental changes in mouse offspring." dans: Particle and fibre toxicology, Vol. 15, Issue 1, pp. 36, (2018) (PubMed).

Onoda, Takeda, Umezawa: "Dose-dependent induction of astrocyte activation and reactive astrogliosis in mouse brain following maternal exposure to carbon black nanoparticle." dans: Particle and fibre toxicology, Vol. 14, Issue 1, pp. 4, (2017) (PubMed).

Onoda, Kawasaki, Tsukiyama, Takeda, Umezawa: "Perivascular Accumulation of β-Sheet-Rich Proteins in Offspring Brain following Maternal Exposure to Carbon Black Nanoparticles." dans: Frontiers in cellular neuroscience, Vol. 11, pp. 92, (2017) (PubMed).

Nishino, Sasaki, Chihara, Nagasaki, Torigoe, Kon, Agui: "Distinct distribution of the tensin family in the mouse kidney and small intestine." dans: Experimental animals, Vol. 61, Issue 5, pp. 525-32, (2013) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.