Âne anti-Chévre IgG Anticorps (DyLight 680) - Preadsorbed

N° du produit ABIN6698876

N° du produit (Fournisseur): 605-744-125

Aperçu rapide pour Âne anti-Chévre IgG Anticorps (DyLight 680) - Preadsorbed (ABIN6698876)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: DyLight 680

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Détail du produit

Reactivité: Chévre

Hôte: Âne

N° du produit (Fournisseur): 605-744-125

Fournisseur: Rockland

Fonction: Goat IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Ch GP Ham Hs Ms Rb & Rt Serum Proteins

Attributs du produit: Donkey anti-Goat IgG Antibody DyLight™ 680 Conjugated Pre-Adsorbed, Donkey anti-Goat IgG DyLight™ 680 Conjugated Antibody,Anti-Goat IgG DyLight Antibody generated in donkey detects goat IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogène: Goat IgG whole molecule

Labeling Ratio: 3.4

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Commentaires:

Anti-Goat IgG DyLight 680 Antibody has been tested by western blot. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Van den Hoecke, Ballegeer, Vrancken, Deng, Job, Roose, Schepens, Van Hoecke, Lemey, Saelens: "In Vivo Therapy with M2e-Specific IgG Selects for an Influenza A Virus Mutant with Delayed Matrix Protein 2 Expression." dans: mBio, Vol. 12, Issue 4, pp. e0074521, (2021) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.