Chèvre anti-Hamster arménien IgG Anticorps (DyLight 405) - Preadsorbed

N° du produit ABIN6698889

N° du produit (Fournisseur): 620-146-440

Aperçu rapide pour Chèvre anti-Hamster arménien IgG Anticorps (DyLight 405) - Preadsorbed (ABIN6698889)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: DyLight 405

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Détail du produit

Reactivité: Hamsters dorés syriens, Hamster arménien

Hôte: Chèvre

N° du produit (Fournisseur): 620-146-440

Fournisseur: Rockland

Fonction: Golden Syrian & Armenian Hamster IgG (H&L) Antibody DyLight™ 405 Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Mouse and Rat Serum Proteins

Attributs du produit: goat anti-Golden Syrian & Armenian Hamster IgG DyLight™405 conjugated antibody, goat anti-Hamster IgG DyLight™ 405 conjugated antibody,Anti-Golden Syrian & Armenian Hamster IgG DyLight Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogène: Armenian and Golden Syrian Hamster IgG, whole molecule

Labeling Ratio: 2.0

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Commentaires:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: FC

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Hirve, Levytskyy, Rigaud, Guimond, Zal, Sauer, Tsoukas: "A conserved motif in the ITK PH-domain is required for phosphoinositide binding and TCR signaling but dispensable for adaptor protein interactions." dans: PLoS ONE, Vol. 7, Issue 9, pp. e45158, (2013) (PubMed).

Levytskyy, Hirve, Guimond, Min, Andreotti, Tsoukas: "In Vivo Consequences of Disrupting SH3-Mediated Interactions of the Inducible T-Cell Kinase." dans: Journal of signal transduction, Vol. 2012, pp. 694386, (2012) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.