Âne anti-Souris IgG Anticorps (DyLight 680) - Preadsorbed

N° du produit ABIN6699034

N° du produit (Fournisseur): 610-744-124

Aperçu rapide pour Âne anti-Souris IgG Anticorps (DyLight 680) - Preadsorbed (ABIN6699034)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: DyLight 680

Application: ELISA, Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Détail du produit

Reactivité: Souris

Hôte: Âne

N° du produit (Fournisseur): 610-744-124

Fournisseur: Rockland

Fonction: Mouse IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins

Attributs du produit: Donkey anti-Mouse IgG DyLight 680™ Conjugated Antibody, Donkey anti Mouse IgG Antibody DyLight 680™ Conjugation,Anti-Mouse IgG DyLight680 Antibody generated in donkey detects reactivity to Mouse IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogène: Mouse IgG whole molecule

Labeling Ratio: 2.0

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Commentaires:

Anti-Mouse IgG DyLight680 Antibody has been tested by ELISA and dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, WB

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Tehrani, Mikulski, Abdul-Zani, Mata, Siwek, Jansen: "STAT1 is required to establish but not maintain interferon-γ-induced transcriptional memory." dans: The EMBO journal, Vol. 42, Issue 14, pp. e112259, (2023) (PubMed).

van den Berg, East, Mitra, Jansen: "p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A." dans: Molecular biology of the cell, Vol. 34, Issue 5, pp. br6, (2023) (PubMed).

Fritz, Ranganathan, Wang, Hogg: "An alternative UPF1 isoform drives conditional remodeling of nonsense-mediated mRNA decay." dans: The EMBO journal, Vol. 41, Issue 10, pp. e108898, (2022) (PubMed).

Navarro, Tapia-Galisteo, Martín-García, Tarín, Corbacho, Gómez-López, Sánchez-Tirado, Campuzano, González-Cortés, Yáñez-Sedeño, Compte, Álvarez-Vallina, Sanz: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." dans: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).

Raimo, Zura-Miller, Fezelinia, Spruce, Zakopoulos, Mohsen, Vockley, Ischiropoulos: "Mitochondrial morphology, bioenergetics and proteomic responses in fatty acid oxidation disorders." dans: Redox biology, Vol. 41, pp. 101923, (2021) (PubMed).

Siwek, Tehrani, Mata, Jansen: "Activation of Clustered IFNγ Target Genes Drives Cohesin-Controlled Transcriptional Memory." dans: Molecular cell, Vol. 80, Issue 3, pp. 396-409.e6, (2021) (PubMed).

Bobkov, Huang, van den Berg, Mitra, Anselm, Lazou, Schunter, Feederle, Imhof, Lusser, Jansen, Heun: "Spt6 is a maintenance factor for centromeric CENP-A." dans: Nature communications, Vol. 11, Issue 1, pp. 2919, (2020) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.