Chèvre anti-Lapin IgG Anticorps (Cy2) - Preadsorbed

N° du produit ABIN6699089

N° du produit (Fournisseur): 611-111-122

Aperçu rapide pour Chèvre anti-Lapin IgG Anticorps (Cy2) - Preadsorbed (ABIN6699089)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: Cy2

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Détail du produit

Reactivité: Lapin

Hôte: Chèvre

N° du produit (Fournisseur): 611-111-122

Fournisseur: Rockland

Fonction: Rabbit IgG (H&L) Antibody CY2 Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Attributs du produit: Goat Anti Rabbit IgG Antibody CY2 Conjugation, Goat Anti-Rabbit IgG CY2 Conjugated Antibody,Anti-Rabbit IgG (H&L) Antibody CY2 Conjugated generated in goat detects reactivity to Rabbit IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogène: Rabbit IgG whole molecule

Labeling Ratio: 4.5

Information d'application

Indications d'application:

FLISA_Dilution: 1:10,000 - 1:50,000

ELISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Western_Blot_Dilution: User Optimized

Commentaires:

Anti-Rabbit IgG (H&L) Conjugated Cy2 Secondary Antibody has been tested by dot blot and western bot. This antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF, Multiplex

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Renko, Tolonen, Rysä, Magga, Mustonen, Ruskoaho, Serpi: "SDF1 gradient associates with the distribution of c-Kit+ cardiac cells in the heart." dans: Scientific reports, Vol. 8, Issue 1, pp. 1160, (2018) (PubMed).

Venkatesan, Natarajan, Schwarz, Mayer, Alpadi, Magupalli, Sung, Schmitz: "Nicotinamide adenine dinucleotide-dependent binding of the neuronal Ca2+ sensor protein GCAP2 to photoreceptor synaptic ribbons." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 19, pp. 6559-76, (2010) (PubMed).

Pyo, Sui, Dhume, Palomeque, Blaxall, Diaz, Tunstead, Logothetis, Hajjar, Schecter: "CXCR4 modulates contractility in adult cardiac myocytes." dans: Journal of molecular and cellular cardiology, Vol. 41, Issue 5, pp. 834-44, (2007) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.