Âne anti-Lapin IgG Anticorps (DyLight 549) - Preadsorbed

N° du produit ABIN6699106

N° du produit (Fournisseur): 611-742-127

Aperçu rapide pour Âne anti-Lapin IgG Anticorps (DyLight 549) - Preadsorbed (ABIN6699106)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: DyLight 549

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Détail du produit

Reactivité: Lapin

Hôte: Âne

N° du produit (Fournisseur): 611-742-127

Fournisseur: Rockland

Fonction: Rabbit IgG (H&L) Antibody DyLight™ 549 Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Attributs du produit: Donkey Anti-Rabbit IgG Antibody DyLight 549™ Conjugated, Donkey Anti Rabbit IgG DyLight 549™ Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 549 Antibody generated in donkey detects reactivity to Rabbit IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogène: Rabbit IgG whole molecule

Labeling Ratio: 3.0

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Commentaires:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Anti-Rabbit IgG (H&L) DyLight 549 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF, IHC, Multiplex

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Hirano, Suzuki, Ikenaka, Hoshi, Tabuchi: "Neurotoxicity of a pyrethroid pesticide deltamethrin is associated with the imbalance in proteolytic systems caused by mitophagy activation and proteasome inhibition." dans: Toxicology and applied pharmacology, Vol. 430, pp. 115723, (2021) (PubMed).

Hirano, Minagawa, Furusawa, Yunoki, Ikenaka, Yokoyama, Hoshi, Tabuchi: "Growth and neurite stimulating effects of the neonicotinoid pesticide clothianidin on human neuroblastoma SH-SY5Y cells." dans: Toxicology and applied pharmacology, Vol. 383, pp. 114777, (2020) (PubMed).

Bennien, Fischer, Geyer: "Rare genetic variants in the sodium-dependent organic anion transporter SOAT (SLC10A6): Effects on transport function and membrane expression." dans: The Journal of steroid biochemistry and molecular biology, Vol. 179, pp. 26-35, (2019) (PubMed).

Okugawa, Toiyama, Ichikawa, Kawamura, Yasuda, Fujikawa, Saigusa, Ohi, Araki, Tanaka, Inoue, Tanaka, Miki, Kusunoki: "Colony-stimulating factor-1 and colony-stimulating factor-1 receptor co-expression is associated with disease progression in gastric cancer." dans: International journal of oncology, Vol. 53, Issue 2, pp. 737-749, (2018) (PubMed).

Wang, Wang, Liu, Huang, Liu, Xia, Brecha, Pu, Gao: "Protective Effect of ALA in Crushed Optic Nerve Cat Retinal Ganglion Cells Using a New Marker RBPMS." dans: PLoS ONE, Vol. 11, Issue 8, pp. e0160309, (2017) (PubMed).

Nakata, Matsui, Kobayashi, Kobayashi, Anzai: "Organic cation transporter 2 (SLC22A2), a low-affinity and high-capacity choline transporter, is preferentially enriched on synaptic vesicles in cholinergic neurons." dans: Neuroscience, Vol. 252, pp. 212-21, (2014) (PubMed).

Ramamoorthy, Shi: "Ischemia induces different levels of hypoxia inducible factor-1α protein expression in interneurons and pyramidal neurons." dans: Acta neuropathologica communications, Vol. 2, pp. 51, (2014) (PubMed).

Luga, Zhang, Viloria-Petit, Ogunjimi, Inanlou, Chiu, Buchanan, Hosein, Basik, Wrana: "Exosomes mediate stromal mobilization of autocrine Wnt-PCP signaling in breast cancer cell migration." dans: Cell, Vol. 151, Issue 7, pp. 1542-56, (2012) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.