Âne anti-Lapin IgG Anticorps (DyLight 680) - Preadsorbed

N° du produit ABIN6699117

N° du produit (Fournisseur): 611-744-127

Aperçu rapide pour Âne anti-Lapin IgG Anticorps (DyLight 680) - Preadsorbed (ABIN6699117)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: DyLight 680

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Détail du produit

Reactivité: Lapin

Hôte: Âne

N° du produit (Fournisseur): 611-744-127

Fournisseur: Rockland

Fonction: Rabbit IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Attributs du produit: Donkey Anti-Rabbit IgG Antibody DyLight 680™ Conjugated, Donkey Anti Rabbit IgG DyLight 680™ Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 680 Antibody generated in donkey detects reactivity to Rabbit IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogène: Rabbit IgG whole molecule

Labeling Ratio: 2.8

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Commentaires:

Anti-Rabbit IgG (H&L) DyLight 680 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: WB

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

van den Berg, East, Mitra, Jansen: "p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A." dans: Molecular biology of the cell, Vol. 34, Issue 5, pp. br6, (2023) (PubMed).

Conway, Rockhold, SantaCruz-Calvo, Zukowski, Pugh, Hasturk, Kern, Nikolajczyk, Bharath: "Obesity and Fatty Acids Promote Mitochondrial Translocation of STAT3 Through ROS-Dependent Mechanisms." dans: Frontiers in aging, Vol. 3, pp. 924003, (2022) (PubMed).

Tucker, Alicea Pauneto, Barnett, Coleman: "Chronic Ethanol Causes Persistent Increases in Alzheimer's Tau Pathology in Female 3xTg-AD Mice: A Potential Role for Lysosomal Impairment." dans: Frontiers in behavioral neuroscience, Vol. 16, pp. 886634, (2022) (PubMed).

Mendenhall, Liu, Portley, O'Mard, Fattah, Szabo, Bugge, Khillan, Leppla, Moayeri: "Anthrax lethal factor cleaves regulatory subunits of phosphoinositide-3 kinase to contribute to toxin lethality." dans: Nature microbiology, Vol. 5, Issue 12, pp. 1464-1471, (2021) (PubMed).

Coleman, Zou, Qin, Crews: "HMGB1/IL-1β complexes regulate neuroimmune responses in alcoholism." dans: Brain, behavior, and immunity, Vol. 72, pp. 61-77, (2019) (PubMed).

Lin, Chen, Wang, Cai: "Emodin promotes the arrest of human lymphoma Raji cell proliferation through the UHRF1‑DNMT3A‑∆Np73 pathways." dans: Molecular medicine reports, Vol. 16, Issue 5, pp. 6544-6551, (2018) (PubMed).

Coleman, Maile, Jones, Cairns, Crews: "HMGB1/IL-1β complexes in plasma microvesicles modulate immune responses to burn injury." dans: PLoS ONE, Vol. 13, Issue 3, pp. e0195335, (2018) (PubMed).

Chen, Zhang, Xu, Wang, Shi, Xu, Zhang, Wang, Li: "HOXC6 promotes gastric cancer cell invasion by upregulating the expression of MMP9." dans: Molecular medicine reports, Vol. 14, Issue 4, pp. 3261-8, (2017) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.