Chèvre anti-Lapin IgG Anticorps (DyLight 488)

N° du produit ABIN6699123

N° du produit (Fournisseur): 611-141-002

Aperçu rapide pour Chèvre anti-Lapin IgG Anticorps (DyLight 488) (ABIN6699123)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: DyLight 488

Application: Western Blotting (WB), Immunofluorescence (IF), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB), Multiplex Assay (MA)

Isotype: IgG

Détail du produit

Reactivité: Lapin

Hôte: Chèvre

N° du produit (Fournisseur): 611-141-002

Fournisseur: Rockland

Fonction: Rabbit IgG (H&L) Secondary Antibody DyLight™ 488 Conjugated

Attributs du produit: Goat anti-Rabbit IgG Antibody DyLight™488 Conjugation, Goat anti-Rabbit IgG DyLight™ 488 Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 488 Antibody generated in goat detects reactivity to Rabbit IgG.

Purification: Conjugated Anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.

Immunogène: Rabbit IgG whole molecule

Labeling Ratio: 3.6

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Commentaires:

Anti-Rabbit IgG (H&L) DyLight 488 Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: FC, IHC, WB

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

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Sanchez, Tonmoy, Park, Morgan: "Development of a Vascularized Human Skin Equivalent with Hypodermis for Photoaging Studies." dans: Biomolecules, Vol. 12, Issue 12, (2022) (PubMed).

Li, Armstrong, Zhao, Cruz-Cosme, Yang, Zhong, Fu, Wang, Yang, Xia, Cheng, Tang: "Zika Virus Infection Downregulates Connexin 43, Disrupts the Cardiomyocyte Gap Junctions and Induces Heart Diseases in A129 Mice." dans: Journal of virology, Vol. 96, Issue 21, pp. e0137322, (2022) (PubMed).

Chen, Yen, Jang, Wang, Huang, Chen, Hsiao, Chang, Chen: "Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells." dans: Scientific reports, Vol. 11, Issue 1, pp. 644, (2021) (PubMed).

Sanchez, Morgan: "Generation of Self-assembled Vascularized Human Skin Equivalents." dans: Journal of visualized experiments : JoVE, Issue 168, (2021) (PubMed).

Jayanthi, Bachhav, Wan, Martinez Legaspi, Segatori: "A platform for post-translational spatiotemporal control of cellular proteins." dans: Synthetic biology (Oxford, England), Vol. 6, Issue 1, pp. ysab002, (2021) (PubMed).

Tian, Tian, Liu, Dong, Wang, Liu, Zhang, Chen: "Effects of TAZ on human dental pulp stem cell proliferation and migration." dans: Molecular medicine reports, Vol. 15, Issue 6, pp. 4326-4332, (2018) (PubMed).

Dufour, Gillet, Frankel, Weibel, Emonet: "Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." dans: PLoS computational biology, Vol. 12, Issue 9, pp. e1005041, (2017) (PubMed).

"Correction: Direct Correlation between Motile Behavior and Protein Abundance in Single Cells." dans: PLoS computational biology, Vol. 12, Issue 9, pp. e1005149, (2016) (PubMed).

Yang, Wong, Teng, Liu, Lu, Liang, Wei: "Focused ultrasound and interleukin-4 receptor-targeted liposomal doxorubicin for enhanced targeted drug delivery and antitumor effect in glioblastoma multiforme." dans: Journal of controlled release : official journal of the Controlled Release Society, Vol. 160, Issue 3, pp. 652-8, (2012) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.