Chèvre anti-Rat IgG Anticorps (Cy3) - Preadsorbed

N° du produit ABIN6699132

N° du produit (Fournisseur): 612-104-120

Aperçu rapide pour Chèvre anti-Rat IgG Anticorps (Cy3) - Preadsorbed (ABIN6699132)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: Cy3

Application: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotype: IgG

Détail du produit

Reactivité: Rat

Hôte: Chèvre

N° du produit (Fournisseur): 612-104-120

Fournisseur: Rockland

Fonction: Rat IgG (H&L) Antibody CY3 Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rb & Sh Serum Proteins

Attributs du produit: Goat Anti Rat IgG CY3 Conjugated Antibody, Goat Anti-Rat IgG Antibody CY3 Conjugation,Anti-Rat IgG (H&L) CY3 Antibody generated in goat detects reactivity to Rat IgG.

Purification: Preadsorption: Pre-Adsorbed

Immunogène: Rat IgG whole molecule

Labeling Ratio: 9.8

Information d'application

Indications d'application:

FLISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Commentaires:

Anti-Rat IgG (H&L) CY3 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Suggested Applications: IHC

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Arzate, Guerra-Crespo, Covarrubias: "Induction of typical and atypical neurogenesis in the adult substantia nigra after mouse embryonic stem cells transplantation." dans: Neuroscience, Vol. 408, pp. 308-326, (2019) (PubMed).

Torigoe, Yamauchi, Kimura, Uemura, Murakami: "Evidence That the Laminar Fate of LGE/CGE-Derived Neocortical Interneurons Is Dependent on Their Progenitor Domains." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 36, Issue 6, pp. 2044-56, (2016) (PubMed).

Torigoe, Yamauchi, Zhu, Kobayashi, Murakami: "Association of astrocytes with neurons and astrocytes derived from distinct progenitor domains in the subpallium." dans: Scientific reports, Vol. 5, pp. 12258, (2016) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG is conjugated to CY3.