Chèvre anti-Rat IgG Anticorps (DyLight 680)

N° du produit ABIN6699161

N° du produit (Fournisseur): 612-144-002

Aperçu rapide pour Chèvre anti-Rat IgG Anticorps (DyLight 680) (ABIN6699161)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: DyLight 680

Application: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotype: IgG

Détail du produit

Reactivité: Rat

Hôte: Chèvre

N° du produit (Fournisseur): 612-144-002

Fournisseur: Rockland

Fonction: Rat IgG (H&L) Antibody Dylight™ 680 Conjugated

Attributs du produit: Goat Anti-Rat IgG DyLight 680™ Conjugated Antibody, Goat Anti-Rat IgG Antibody DyLight 680™ Conjugation,

Purification:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. This antibody will react with heavy chains of Rat IgG and with light chains of most Rat immunoglobulins.

Immunogène: Rat IgG, whole molecule

Labeling Ratio: 3.0

Information d'application

Indications d'application:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Commentaires:

This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Anti-Rat IgG DyLight™ 680 has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Szewczyk, Günther, Japtok, Frech, Naumann, Lee, Hermann: "FUS ALS neurons activate major stress pathways and reduce translation as an early protective mechanism against neurodegeneration." dans: Cell reports, Vol. 42, Issue 2, pp. 112025, (2023) (PubMed).

Link, Chung, Jolly, Withers, Tepe, Arenkiel, Shah, Krogan, Aydin, Geckinli, Tos, Isikay, Tuysuz, Mochida, Thomas, Clark, Mirzaa, Lupski, Bellen: "Mutations in ANKLE2, a ZIKA Virus Target, Disrupt an Asymmetric Cell Division Pathway in Drosophila Neuroblasts to Cause Microcephaly." dans: Developmental cell, Vol. 51, Issue 6, pp. 713-729.e6, (2020) (PubMed).

Bacman, Kauppila, Pereira, Nissanka, Miranda, Pinto, Williams, Larsson, Stewart, Moraes: "MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation." dans: Nature medicine, Vol. 24, Issue 11, pp. 1696-1700, (2019) (PubMed).

Bacman, Kauppila, Pereira, Nissanka, Miranda, Pinto, Williams, Larsson, Stewart, Moraes: "Author Correction: MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation." dans: Nature medicine, Vol. 24, Issue 12, pp. 1940, (2018) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody