Recombinant Alpaca anti-Poulet IgY Anticorps (Alexa Fluor 647)

N° du produit ABIN7272912

Aperçu rapide pour Recombinant Alpaca anti-Poulet IgY Anticorps (Alexa Fluor 647) (ABIN7272912)

Antigène: IgY

Type d'anticorp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Clonalité: Monoclonal

Conjugué: Alexa Fluor 647

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 3G2

Détail du produit

Reactivité: Poulet

Hôte: Alpaca

Expression System: E.coli

Fonction: Camelid sdAb anti-Chicken IgY Alexa Fluor™ 647, Clone(s) 3G2

Specificité: Chicken IgY, does not cross-react with mouse, rabbit, guinea pig or rat immunglobulins

Purification: Produced in: E.coli

Immunogène: anti-Chicken is a species-specific camelid sdAB directed against immunoglobulins from Chicken.

Labeling Ratio: Two site-specifically conjugated fluorophores per sdAb.

Information d'application

Indications d'application: IF: 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed bellow)

Commentaires:

Two site-specifically coupled fluorophores per molecule. The reagent can therefore simultaneously target two fluorophores to your protein of interest, which results in enhanced image brightness. Owing to the small size of the sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this sdAb can thus improve the localization accuracy by 10-15 nm. Both features - enhanced brightness and precise fluorophore placement - renders this product superior tools for all microscopy techniques.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM

Buffer: The single sdAb clone was lyophilized from PBS pH 7.4.

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.

Détail du antigène

Antigène: IgY

Classe de substances: Antibody

Sujet: The immune repertoire of chickens is much simpler compared to the one of mammals, producing only one isotype of immunoglobulins (IgY) with no subclasses. The use of polyclonal antibodies raised in chicken has steadily grown over the last years, mainly due to the need to add a new “species” when performing multitarget immunofluorescence.