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Souris anti-Humain IgA2 (Allotype A2m2) Anticorps

ELISA, IFA Monoclonal NI 194-3 (A89-040) IgG1 unconjugated
N° du produit ABIN7674781

Aperçu rapide pour Souris anti-Humain IgA2 (Allotype A2m2) Anticorps (ABIN7674781)

Antigène

IgA2

Clonalité

  • 17
  • 2
Monoclonal

Application

  • 16
  • 8
  • 8
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
ELISA, Indirect Immunofluorescence Assay (IFA)

Clone

NI 194-3 (A89-040)

Isotype

IgG1
  • Épitope

    • 5
    • 4
    • 4
    • 1
    • 1
    Allotype A2m2

    Reactivité

    Humain

    Hôte

    • 17
    • 2
    Souris

    Fonction

    Purified monoclonal Mouse antibody to Human IgA2(m)2, allotype specific

    Specificité

    Purified monoclonal Mouse antibody to Human IgA2(m)2, allotype specific

    Réactivité croisée (Details)

    The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

    Attributs du produit

    The reactivity of the antiserum is restricted to the IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.

    Purification

    Purified

    Immunogène

    Highly purified monoclonal IgA2(m)2 isolated from human serum.
  • Indications d'application

    Indirect immunofluorescence,ELISA.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline ( pH 7.2) IgG concentration is 0.4 mg/mL. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.

    Buffer

    Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline ( pH 7.2) IgG concentration is 0.4 mg/mL. No foreign proteins added.

    Agent conservateur

    Without preservative

    Stock

    4 °C,-20 °C

    Stockage commentaire

    The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
  • Antigène

    IgA2
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