ELISA, IFA
Monoclonal
NI 194-3 (A89-040)
IgG1
unconjugated
N° du produit ABIN7674781
Aperçu rapide pour Souris anti-Humain IgA2 (Allotype A2m2) Anticorps (ABIN7674781)
Antigène
IgA2
Clonalité
Monoclonal
Application
ELISA, Indirect Immunofluorescence Assay (IFA)
Clone
NI 194-3 (A89-040)
Isotype
IgG1
Épitope
Allotype A2m2
Reactivité
Humain
Hôte
Souris
Fonction
Purified monoclonal Mouse antibody to Human IgA2(m)2, allotype specific
Specificité
Purified monoclonal Mouse antibody to Human IgA2(m)2, allotype specific
Réactivité croisée (Details)
The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
Attributs du produit
The reactivity of the antiserum is restricted to the IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.
Purification
Purified
Immunogène
Highly purified monoclonal IgA2(m)2 isolated from human serum.
ELISA, WB, ICC, IHC (p), DB
Monoclonal
NI 194-3 (A89-040)
IgG1
HRP
Indications d'application
Indirect immunofluorescence,ELISA.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline ( pH 7.2) IgG concentration is 0.4 mg/mL. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.
Buffer
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline ( pH 7.2) IgG concentration is 0.4 mg/mL. No foreign proteins added.
Agent conservateur
Without preservative
Stock
4 °C,-20 °C
Stockage commentaire
The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.