ELISA, IFA
Monoclonal
NI 86 (HP 6080)
IgG1
unconjugated
N° du produit ABIN7674787
Aperçu rapide pour Souris anti-Humain IgG3 (Allotype G3m(U)) Anticorps (ABIN7674787)
Antigène
IgG3
Clonalité
Monoclonal
Application
ELISA, Indirect Immunofluorescence Assay (IFA)
Clone
NI 86 (HP 6080)
Isotype
IgG1
Épitope
Allotype G3m(U)
Reactivité
Humain
Hôte
Souris
Fonction
Monoclonal Mouse antibody to Human IgG3m(u), allotype specific.
Specificité
Monoclonal Mouse antibody to Human IgG3m(u), allotype specific.
Réactivité croisée (Details)
The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.
Attributs du produit
The reactivity of the antiserum is restricted to an allotype specific determinant of the IgG3m(u) molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3m(u) in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3m(u), and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Purification
Purified
Immunogène
Highly purified monoclonal IgG3m(u) isolated from human serum
ELISA, IFA
Monoclonal
NI 86 (HP 6080)
IgG1
unconjugated
Indications d'application
Indirect immunofluorescence,ELISA.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/mL. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
Buffer
Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration is 0.4 mg/mL. No foreign proteins added.
Agent conservateur
Without preservative
Stock
4 °C,-20 °C
Stockage commentaire
The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.