Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps (HRP) - Preadsorbed

N° du produit ABIN964977

N° du produit (Fournisseur): 611-1322-0100

Aperçu rapide pour Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps (HRP) - Preadsorbed (ABIN964977)

Antigène: IgG

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Lapin

Hôte: Chèvre

N° du produit (Fournisseur): 611-1322-0100

Fournisseur: Rockland

Fonction: Rabbit IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Human Serum Proteins.

Attributs du produit: Anti-Rabbit IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the rabbit IgG molecule. Rabbit IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind rabbit IgG F(c) will not bind rabbit IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG F(ab')2 is conjugated to Peroxidase.

Purification: Anti-RABBIT IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène:

Immunogen: Rabbit IgG whole molecule

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Goat Anti-Rabbit IgG peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:750 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:50,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Référence

Cong, Gong, Yang, Xia, Zhang: "miR-22 Suppresses Tumor Invasion and Metastasis in Colorectal Cancer by Targeting NLRP3." dans: Cancer management and research, Vol. 12, pp. 5419-5429, (2020) (PubMed).

Nuwer, Fleck: "Anterograde trafficking signals in GABAA subunits are required for functional expression." dans: Channels (Austin, Tex.), Vol. 13, Issue 1, pp. 440-454, (2020) (PubMed).

Agrawal, Lehtonen, Uusi-Mäkelä, Jain, Viitala, Määttä, Kähkönen, Azizi, Riihimäki, Kulomaa, Johnson, Hytönen, Airenne: "Molecular features of steroid-binding antidins and their use for assaying serum progesterone." dans: PLoS ONE, Vol. 14, Issue 2, pp. e0212339, (2019) (PubMed).

Shrestha, Ahn, Staples, Sathyan, Karpova, Foltz, Basrai: "Mislocalization of centromeric histone H3 variant CENP-A contributes to chromosomal instability (CIN) in human cells." dans: Oncotarget, Vol. 8, Issue 29, pp. 46781-46800, (2018) (PubMed).

Millonig, Ganzleben, Peccerella, Casanovas, Brodziak-Jarosz, Breitkopf-Heinlein, Dick, Seitz, Muckenthaler, Mueller: "Sustained submicromolar H2O2 levels induce hepcidin via signal transducer and activator of transcription 3 (STAT3)." dans: The Journal of biological chemistry, Vol. 287, Issue 44, pp. 37472-82, (2013) (PubMed).

Grando, Bystryn, Chernyavsky, Frusić-Zlotkin, Gniadecki, Lotti, Milner, Pittelkow, Pincelli: "Apoptolysis: a novel mechanism of skin blistering in pemphigus vulgaris linking the apoptotic pathways to basal cell shrinkage and suprabasal acantholysis." dans: Experimental dermatology, Vol. 18, Issue 9, pp. 764-70, (2009) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Anti-Rabbit IgG peroxidase conjugated antibody generated in goat detects specifically rabbit IgG. Both the Heavy and Light chains of the antibody molecule are present. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG is conjugated with Peroxidase. This peroxidase conjugated anti-Rabbit secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.