Lapin anti-Chévre IgG (Heavy & Light Chain) Anticorps (PE)

N° du produit ABIN965039

N° du produit (Fournisseur): 705-408-002

Aperçu rapide pour Lapin anti-Chévre IgG (Heavy & Light Chain) Anticorps (PE) (ABIN965039)

Antigène: IgG

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Conjugué: PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Chévre

Hôte: Lapin

N° du produit (Fournisseur): 705-408-002

Fournisseur: Rockland

Fonction: F(ab')2 Goat IgG (H&L) Antibody Phycoerythrin conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Goat IgG and Goat Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Attributs du produit: F(ab')2 Anti-Goat IgG Biotin Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: F(ab')2 fragment antibody Anti-Goat IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.  Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody.

Immunogène: Optional[Immunogen]: Goat IgG whole molecule

Information d'application

Indications d'application: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.