Chèvre anti-Cobaye IgG (Heavy & Light Chain) Anticorps (TRITC)
Aperçu rapide pour Chèvre anti-Cobaye IgG (Heavy & Light Chain) Anticorps (TRITC) (ABIN965054)
Antigène
Fragment
Clonalité
Conjugué
Application
Isotype
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Épitope
- Heavy & Light Chain
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Reactivité
- Cobaye
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Hôte
- Chèvre
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N° du produit (Fournisseur)
- 706-100-002
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Fournisseur
- Rockland
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Fonction
- F(ab')2 Guinea Pig IgG (H&L) Antibody Rhodamine Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
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Attributs du produit
- F(ab')2 Anti-Goat IgG Texas Red Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.
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Purification
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Guinea Pig IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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Immunogène
- Optional[Immunogen]: Guinea Pig IgG whole molecule
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Indications d'application
- Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
, Preservative:0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- IgG
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Classe de substances
- Antibody
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Sujet
- Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
Antigène
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