Lapin anti-Cheval IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN965073

N° du produit (Fournisseur): 708-405-002

Aperçu rapide pour Lapin anti-Cheval IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) (ABIN965073)

Antigène: IgG

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Cheval

Hôte: Lapin

N° du produit (Fournisseur): 708-405-002

Fournisseur: Rockland

Fonction: F(ab')2 Horse IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Rabbit Serum, Horse IgG and Horse Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Attributs du produit: F(ab')2 Anti-Horse IgG Peroxidase Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Horse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Horse IgG whole molecule

Information d'application

Indications d'application: Application Note: F(ab')2 Anti-Horse IgG Alkaline Phosphatase Antibody has been tested by ELISA and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Horse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:250-1:500 Western Blot Dilution: 1:500-1:1,000 ELISA Dilution: 1:1,000 - 1:15,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Optional[Buffer]: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.