Âne anti-Souris IgG (Heavy & Light Chain) Anticorps (TRITC) - Preadsorbed

N° du produit ABIN965199

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Âne

Clonalité: Polyclonal

Conjugué: TRITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.

Attributs du produit: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Mouse IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Conseil sur la manipulation: Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4 °C prior to opening. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Donkey F(ab')2 Anti-Mouse IgG Antibody rhodamine Conjugation, Donkey Fab2 Anti-Mouse IgG TRITC Conjugated Antibody

Background: F(ab')2 Anti-Mouse IgG (H&L) Rhodamine Antibody generated in donkey was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.