Chèvre anti-Lapin IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

N° du produit ABIN965222

N° du produit (Fournisseur): 711-1524

Aperçu rapide pour Chèvre anti-Lapin IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed (ABIN965222)

Antigène: IgG

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: F(ab')2 Region

Reactivité: Lapin

Hôte: Chèvre

N° du produit (Fournisseur): 711-1524

Fournisseur: Rockland

Fonction: F(ab')2 Rabbit IgG F(ab')2 Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Hs Hu Ms Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab')2   and Rabbit Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rat and Sheep Serum Proteins.

Attributs du produit: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Rabbit IgG F(ab')2 fragment

Information d'application

Indications d'application: Application Note: F(ab')2 Anti-Rabbit IgG F(ab')2 Alkaline Phosphatase Antibody has been tested by ELISA and is assayed against 1.0 μg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:5,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: F(ab')2 Anti-Rabbit IgG F(ab')2 Alkaline Phosphatase Antibody generated in goat detects Rabbit F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.