Chèvre anti-Rat IgG (Fc Region) Anticorps - Preadsorbed

N° du produit ABIN965245

N° du produit (Fournisseur): 712-1122

Aperçu rapide pour Chèvre anti-Rat IgG (Fc Region) Anticorps - Preadsorbed (ABIN965245)

Antigène: IgG

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Fc Region

Reactivité: Rat

Hôte: Chèvre

N° du produit (Fournisseur): 712-1122

Fournisseur: Rockland

Fonction: F(ab')2 Rat IgG Fc Antibody Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum.  No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Rat IgG F(ab)  or Bovine, Horse and Human Serum Proteins.

Attributs du produit: F(ab')2 Anti-Rat IgG (H&L) Antibody generated in goat detects rat IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Rat IgG Antibody is conjugated to Texas Red™.

Purification: Anti-Rat F(c) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Anti-Rat IgG F(c) was produced by repeated immunization with Rat F(c) fragment in goat.

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: F(ab')2 Anti-Rat IgG F(c) Antibody is suitable for immunoblotting, western blot, dot blot, ELISA, and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:1,000 - 1:3,000 Western Blot Dilution: 1:2,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: F(ab')2 Anti-Rat IgG F(c) Antibody was generated in goat and detects specifically Rat IgG F(c). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.