Âne anti-Mouton IgG (Heavy & Light Chain) Anticorps (TRITC) - Preadsorbed

N° du produit ABIN965287

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Épitope: Heavy & Light Chain

Reactivité: Mouton

Hôte: Âne

Clonalité: Polyclonal

Conjugué: TRITC

Application: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Sheep IgG and Sheep Serum.

Attributs du produit: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Sheep IgG whole molecule

Isotype: IgG

Labeling Ratio: 2.4

Information d'application

Indications d'application:

Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

FLISA Dilution: 1:10,000 - 1:50,000

Flow Cytometry Dilution: 1:500 - 1:2,500

IF Microscopy Dilution: 1:1,000 - 1:5,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20 °C or below prior to opening. Store the vial at -20 °C or below after dilution.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Donkey F(ab')2 Anti-Sheep IgG Antibody Rhodamine Conjugation, Donkey F(ab')2 Anti-Sheep IgG TRITC Conjugated Antibody

Background: F(ab')2 Anti-Sheep IgG (H&L) Antibody generated in donkey detects sheep IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This F(ab')2 Anti-Sheep IgG Antibody is conjugated to rhodamine.