Lapin anti-Porc IgG (Heavy & Light Chain) Anticorps (PE)

N° du produit ABIN965298

N° du produit (Fournisseur): 714-408-002

Aperçu rapide pour Lapin anti-Porc IgG (Heavy & Light Chain) Anticorps (PE) (ABIN965298)

Antigène: IgG

Fragment: F(ab')2 fragment

Clonalité: Polyclonal

Conjugué: PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Porc

Hôte: Lapin

N° du produit (Fournisseur): 714-408-002

Fournisseur: Rockland

Fonction: F(ab')2 Swine IgG (H&L) Antibody Phycoerythrin conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-phycoerythrin, anti-Rabbit Serum, Swine IgG and Swine Serum.  No reaction was observed against anti-Pepsin or anti-Rabbit IgG F(c).

Attributs du produit: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogène: Optional[Immunogen]: Swine IgG whole molecule

Information d'application

Indications d'application: Application Note: F(ab')2 Anti-Swine IgG Phycoerythrin Conjugated Secondary Antibody reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: FLOW 1:100 - 1:250

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: F(ab')2 Anti-Swine IgG (H&L) Antibody generated in rabbit detects immunoglobulin g from swine, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. This F(ab')2 Anti-Swine IgG Antibody is conjugated to Phycoerythrin.