Chèvre anti-Souris IgG (Heavy & Light Chain) Anticorps (HRP)

N° du produit ABIN965359

N° du produit (Fournisseur): 810-1302

Aperçu rapide pour Chèvre anti-Souris IgG (Heavy & Light Chain) Anticorps (HRP) (ABIN965359)

Antigène: IgG

Fragment: Fab fragment

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Souris

Hôte: Chèvre

N° du produit (Fournisseur): 810-1302

Fournisseur: Rockland

Fonction: Fab Mouse IgG (H&L) Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).

Attributs du produit: Fab Anti-Mouse IgG (H&L) Fluorescein Antibody generated in goat detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Immunogène:

Immunogen: Anti-Mouse IgG (H&L) was produced by repeated immunization with mouse IgG whole molecule in goat.

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Fab Anti-Mouse IgG (H&L) peroxidase conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:10,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Référence

Goodpaster, Randolph-Habecker: "A flexible mouse-on-mouse immunohistochemical staining technique adaptable to biotin-free reagents, immunofluorescence, and multiple antibody staining." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 62, Issue 3, pp. 197-204, (2014) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Fab Anti-Mouse IgG (H&L) Peroxidase Antibody generated in goat detects Mouse IgG. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.