Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps

N° du produit ABIN965369

N° du produit (Fournisseur): 811-1102

Aperçu rapide pour Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps (ABIN965369)

Antigène: IgG

Fragment: Fab fragment

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Lapin

Hôte: Chèvre

N° du produit (Fournisseur): 811-1102

Fournisseur: Rockland

Fonction: Fab Rabbit IgG (H&L) Antibody

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.  No reaction was observed against anti-Papain or anti-Goat IgG F(c).

Attributs du produit: Fab Anti-Rabbit IgG (H&L) Antibody generated in goat detects rabbit IgG. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Fab Antibody is ideal for investigators who routinely perform flow cytometry, immunofluorescence, IHC, and other immunoassays. This Fab Anti-Rabbit IgG Antibody is conjugated to rhodamine.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

Stérilité: Sterile filtered

Immunogène: Optional[Immunogen]: Rabbit IgG whole molecule

Information d'application

Indications d'application: Application Note: Fab Anti-Rabbit IgG (H&L) Antibody has been tested by dot blot and is suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:4,000 - 1:20,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Dembla, Kesharwani, Natarajan, Fecher-Trost, Fairless, Williams, Flockerzi, Diem, Schwarz, Schmitz: "Early auto-immune targeting of photoreceptor ribbon synapses in mouse models of multiple sclerosis." dans: EMBO molecular medicine, Vol. 10, Issue 11, (2019) (PubMed).

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Fab Anti-Rabbit IgG (H&L) Antibody generated in goat detects immunoglobulin g from rabbit, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.