Âne anti-Lapin IgG (Heavy & Light Chain) Anticorps (Biotin)

N° du produit ABIN965377

N° du produit (Fournisseur): 811-7602

Aperçu rapide pour Âne anti-Lapin IgG (Heavy & Light Chain) Anticorps (Biotin) (ABIN965377)

Antigène: IgG

Fragment: Fab fragment

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Détail du produit

Épitope: Heavy & Light Chain

Reactivité: Lapin

Hôte: Âne

N° du produit (Fournisseur): 811-7602

Fournisseur: Rockland

Fonction: Fab Rabbit IgG (H&L) Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Donkey Serum.  No reaction was observed against anti-Papain or anti-Donkey IgG F(c).

Attributs du produit: Fab Anti-Rabbit IgG (H&L) antibody generated in Donkey detects specifically Rabbit IgG whole molecule. This Fab Anti-Rabbit IgG Secondary Antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Fab Anti-Rabbit IgG (H&L) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.

Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

Immunogène: Optional[Immunogen]: Rabbit IgG whole molecule

Information d'application

Indications d'application: Application Note: Fab Anti-Rabbit IgG Biotin Antibody has been tested by ELISA and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:75,000 - 1:150,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Classe de substances: Antibody

Sujet: Fab Anti-Rabbit IgG (H&L) Antibody generated in donkey detects immunoglobulin g from rabbit, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Fab Anti-Rabbit IgG Antibody is conjugated to Biotin.