Chèvre anti-Humain Ig (Chain kappa), (Light Chain) Anticorps (Alkaline Phosphatase (AP))

N° du produit ABIN969902

Aperçu rapide pour Chèvre anti-Humain Ig (Chain kappa), (Light Chain) Anticorps (Alkaline Phosphatase (AP)) (ABIN969902)

Antigène: Ig

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Détail du produit

Épitope: Chain kappa, Light Chain

Reactivité: Humain

Hôte: Chèvre

Specificité: Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins

Réactivité croisée (Details): Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins

Attributs du produit: Concentration: 1.50 mg/mL (E 1 % at 280 nm = 13.0)
Conjugate: Alkaline Phosphatase

Purification: Affinity purified using solid phase human Kappa (κ) Chain

Pureté: > 95 % based on SDS-PAGE

Immunogène: Purified human kappa (kappa) light chain

Information d'application

Indications d'application: This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Commentaires:

ALP: Enzyme derived from calf intestine (U.S. origin), Catalyzes the hydrolysis of phosphate groups from a substrate, which produces a colored reaction product or a release of light, The most common substrate for ALP is pNPP, which produces a soluble product, Unaffected by biological components in the ELISA assay, and produces consistent results, The reaction rate of ALP is linear, so its detection sensitivity can be increased by increasing the length of the reaction time. Detection OD of 405 nm

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.50 mg/mL

Buffer: 30 mM Triethanolamine, pH 7.2, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Prepare working dilution prior to use and then discard.

Stock: 4 °C

Stockage commentaire: Store undiluted liquid at 2-8 °C. For storage at -20 °C, dilute with an equal volume of glycerol to prevent loss of enzymatic activity. Prepare working dilutions prior to use and then discard.

Détail du antigène

Antigène: Ig

Autre désignation: Immunoglobulin Ig