Human Tumor Tissue Blot III
Aperçu rapide pour Human Tumor Tissue Blot III (ABIN2455970)
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Application
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Attributs du produit
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The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human tumor tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20 % SDS-PAGE gel and transferred onto nitrocellulose membrane.The human tumor tissues included in the blot are bladder, breast, kidney, ovary, prostate, testis, cervix, and uterus.
Sample diagnosis: Tumor
Samples loaded: Lanes: 1: protein markers, 2: bladder, 3: breast, 4: kidney, 5: ovary, 6: prostate, 7: testis, 8: cervix, and 9: uterus.
Quantity loaded per lane: 15 μg
Gel percentage: Gradient 4-20 %
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Indications d'application
- For Western Blot, the blot can be blocked with 1 % BSA or 5 % nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.
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Restrictions
- For Research Use only
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Conseil sur la manipulation
- The blot can be reused after stripping.
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Stock
- 4 °C
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Stockage commentaire
- Store at 4 °C.
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