NR4A1 anticorps (AA 401-414)
Aperçu rapide pour NR4A1 anticorps (AA 401-414) (ABIN337182)
Antigène
Voir toutes NR4A1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 401-414
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Specificité
- Human NR4A1 / NUR77. This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.
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Homologie
- Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Rabbit (100%) Marmoset, Bat (93%) Bovine (86%).
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Purification
- Immunoaffinity purified
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Immunogène
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Synthetic peptide C-GRLPSKPKQPPDAS from an internal region of human NR4A1 / NUR77 (NP_002126.2, NP_775180.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Dog, Horse, Rabbit (100%), Marmoset, Bat (93%), Bovine (86%).
Type of Immunogen: Synthetic peptide
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Indications d'application
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Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Store at -20°C. Avoid freeze-thaw cycles.
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- NR4A1 (Nuclear Receptor Subfamily 4, Group A, Member 1 (NR4A1))
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Autre désignation
- NR4A1 / NUR77
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Sujet
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Name/Gene ID: NR4A1
Subfamily: NR4 Nerve growth factor-like
Family: NHR
Synonyms: NR4A1, GFRP1, Early response protein NAK1, HMR, NGFIB, Orphan nuclear receptor TR3, Orphan receptor tr3, Steroid receptor TR3, TR3, NAK1, NGFI-B, NP10, Receptor NGFIB, Testicular receptor 3, TR3 orphan receptor, N10, NAK-1, NUR77, Orphan nuclear receptor HMR -
ID gène
- 3164
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NCBI Accession
- NP_002126, NP_775180
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UniProt
- P22736
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Pathways
- Fc-epsilon Receptor Signaling Pathway, Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Steroid Hormone Mediated Signaling Pathway
Antigène
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