TSPO anticorps (C-Term)
Aperçu rapide pour TSPO anticorps (C-Term) (ABIN185697)
Antigène
Voir toutes TSPO AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Classe de qualité
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Épitope
- C-Term
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Fonction
- TSPO / PBR (isoform)
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Séquence
- RDNHGWRGGR RLPE
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Specificité
- This antibody is expected to recognise one isoform (NP_000705.2) only.
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Réactivité croisée
- Humain
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Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Immunogène
- Peptide with sequence C-RDNHGWRGGRRLPE, from the C Terminus of the protein sequence according to NP_000705.2.
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Isotype
- IgG
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Indications d'application
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Immunohistochemistry: In paraffin embedded Human Colon shows strong cytoplasm staining of absorptive cells. Recommended concentration: 2-4 μg/mL. Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 17 kDa band observed in Human Breast cancer lysates and approx 16 kDa in Human Ovary and Skin lysates, and in lysates of cell line A431 (calculated MW of 18.8 kDa according to NP_000705.2).These molecular weights are routinely observed
Peptide ELISA: antibody detection limit dilution 1:128000.
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Commentaires
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and MCF7 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- Minimize freezing and thawing.
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Stock
- -20 °C
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Stockage commentaire
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))
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Autre désignation
- TSPO
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Sujet
- TSPO, PBR, translocator protein (18 kDa) , BZRP, DBI, IBP, MBR, PKBS, PTBR, mDRC, pk18 , OTTHUMP00000028891, benzodiazapine receptor (peripheral), benzodiazepine peripheral binding site, mitochondrial benzodiazepine receptor, peripheral benzodiazepine rece
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ID gène
- 706
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NCBI Accession
- NP_000705
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Pathways
- Cellular Response to Molecule of Bacterial Origin
Antigène
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