His Tag anticorps
Aperçu rapide pour His Tag anticorps (ABIN306800)
Antigène
Voir toutes His Tag AnticorpsHôte
Clonalité
Conjugué
Application
Clone
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Fonction
- Mouse anti His Tag
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Séquence
- HHHHHH
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Attributs du produit
- 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags only.
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Purification
- Protein A/G Chromatography
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Isotype
- IgG2a
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anti-His Tag antibody
Reactivité: Humain WB Hôte: Souris Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IHC, DB Hôte: Lapin Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA Hôte: Lapin Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS Hôte: Souris Monoclonal 6G2A9 unconjugated
anti-His Tag antibodyWB, ELISA, IP Hôte: Souris Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibodyReactivité: Humain WB Hôte: Lapin Polyclonal RB19960 unconjugated
anti-His Tag antibody (DyLight 649)WB, ELISA, FM, DB, FLISA Hôte: Souris Monoclonal 33D10-D2-G8 DyLight 649
anti-His Tag antibody (HRP)WB, ELISA, IHC Hôte: Souris Monoclonal 33D10-D2-G8 HRP
anti-His Tag antibody (Cy3)WB, ELISA, FACS, FM, DB, FLISA Hôte: Souris Monoclonal 33D10-D2-G8 Cy3
anti-His Tag antibody (DyLight 800)WB, ELISA, FM, DB, FLISA Hôte: Souris Monoclonal 33D10-D2-G8 DyLight 800
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Indications d'application
- Detects only 6x-His tag by Western blot. Specific for His tags no longer than 6 histidines. Optimal concentration should be evaluated by serial dilutions.
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Restrictions
- For Research Use only
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Stock
- -20 °C
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Stockage commentaire
- Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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- His Tag
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Classe de substances
- Tag
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Sujet
- Anti-6x-Histidine Tag Antibody
Antigène
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