Pour une meilleure utilisation, nous vous conseillons d’activer Javascript dans votre navigateur.

CXCL1 Kit ELISA

Name: CXCL1 Kit ELISA
SKU: ABIN411393
Availability: InStock

Le kit ELISA CXCL1 (ABIN411393) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris CXCL1. Ce kit ELISA a été cité dans 1 publication.

ELISA

1 image

(1 reference)

N° du produit ABIN411393

642,40 €

Plus frais de livraison 40,00 € et TVA

96 tests

Destination: France

Envoi sous 8 à 12 jours ouvrables

Aperçu rapide pour CXCL1 Kit ELISA (ABIN411393)

Antigène

Voir toutes CXCL1 Kits ELISA

CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Épitope

AA 20-96

Reactivité

Souris

Méthode de détection

Colorimetric

Type de méthode

Sandwich ELISA

Gamme de detection

15.6-1000 pg/mL

Application

ELISA

Type d'échantillon

Cell Culture Supernatant, Serum

Discover our top product CXCL1 Kit ELISA

Seuil minimal de détection

15.6 pg/mL

Fonction

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CXCL1

Analytical Method

Quantitative

Specificité

Expression system for standard: E.coli
Immunogen sequence: R20-K96

Réactivité croisée (Details)

There is no detectable cross-reactivity with other relevant proteins.

Sensibilité

<1pg/mL

Matériel non inclus

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène

Expression system for standard: E.coli
Immunogen sequence: R20-K96
Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Humain Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Humain Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Souris Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Rat AA 25-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Humain Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Rat Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Singe Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Rat Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate

Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1) ELISA Kit
CXCL1 Reactivité: Souris Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate

Indications d'application

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque

Pre-coated

Protocole

mouse CXCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CXCL1 has been precoated onto 96-well plates. Standards(E. coli, R20-K96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CXCL1 amount of sample captured in plate.

Procédure de l'essai

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CXCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CXCL1 standard solution and each sample be measured in duplicate.

Précision du teste
- Sample 1: n=16, Mean(pg/ml): 95.7, Standard deviation: 5.84, CV(%): 6.1
- Sample 2: n=16, Mean(pg/ml): 279, Standard deviation: 12.56, CV(%): 4.5
- Sample 3: n=16, Mean(pg/ml): 570, Standard deviation: 36.48, CV(%): 6.4,
- Sample 1: n=24, Mean(pg/ml): 101.3, Standard deviation: 7.29, CV(%): 7.2
- Sample 2: n=24, Mean(pg/ml): 268, Standard deviation: 22.78, CV(%): 8.5
- Sample 3: n=24, Mean(pg/ml): 605, Standard deviation: 41.14, CV(%): 6.8
Restrictions

For Research Use only
Conseil sur la manipulation

Avoid multiple freeze-thaw cycles.

Stock

-20 °C,4 °C

Stockage commentaire

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption

12 months
Wan, Mo, Zhang, Jiang, Tang, Zhang: "Cobalt nanoparticles induce lung injury, DNA damage and mutations in mice." dans: Particle and fibre toxicology, Vol. 14, Issue 1, pp. 38, (2018) (PubMed).
Antigène Voir toutes CXCL1 Kits ELISA

CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))

Autre désignation

CXCL1

Sujet

Background: Chemokine(C-X-C motif) ligand 1(CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.
Synonyms: C-X-C motif chemokine ,Cxcl1 ,mCG_1708 ,
Full Gene Name: C-X-C motif chemokine
Cellular Localisation: Secreted.

ID gène

14825

UniProt

A2RTH0

Pathways

Autophagy