Acetylated Lysine anticorps (acetylated, Lys25)
Aperçu rapide pour Acetylated Lysine anticorps (acetylated, Lys25) (ABIN488677)
Antigène
Reactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- acetylated, Lys25
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Fonction
- Anti-Acetylated lysine (25 μl, clone 7F8)
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Specificité
- The antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.
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Réactivité croisée (Details)
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Bovine, avian
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Homologie
- Higher plants
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Purification
- Protein G purified
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Immunogène
- acetylated KLH
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Isotype
- IgG1
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anti-Acetylated Lysine antibody
Reactivité: Différentes espèces WB, ELISA, IHC, IP, FACS, FM Hôte: Lapin Polyclonal unconjugated
anti-Acetylated Lysine antibodyReactivité: Boeuf (Vache), Poulet WB, ELISA, IHC (p), IHC (fro) Hôte: Souris Monoclonal 7F8 unconjugated
anti-Acetylated Lysine antibodyWB Hôte: Souris Monoclonal 15G10 unconjugated
anti-Acetylated Lysine (acetylated) antibodyWB, IP, IF, EIA Hôte: Lapin Polyclonal unconjugated
anti-Acetylated Lysine (acetylated) antibodyFACS Hôte: Souris Monoclonal 15G10 unconjugated
anti-Acetylated Lysine (acetylated) antibody (PE)FACS Hôte: Souris Monoclonal 15G10 PE
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Indications d'application
- 1 : 100 (IHC), 1 : 1000 (WB)
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Commentaires
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1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Total IgG fraction. Protein G purified.
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Conseil sur la manipulation
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Stock
- -20 °C
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Stockage commentaire
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Acetylated Lysine
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Classe de substances
- Chemical
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Sujet
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Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
Additional information: Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/mLantibody detects Proteins containing acetylated lysine residues in ELISA and WBs, Does not detect non-acetylated lysine residues
Antigène
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