Nav1.8 anticorps (C-Term)
Aperçu rapide pour Nav1.8 anticorps (C-Term) (ABIN492472)
Antigène
Voir toutes Nav1.8 (SCN10A) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
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Épitope
- AA 1724-1956, C-Term
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Specificité
- Detects ~220 kDa protein. No cross reactivity against other Nav channels.
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Attributs du produit
- Synonyms: Sodium channel protein type 10 subunit alpha, Sodium channel protein type X subunitalpha, Voltage-gated sodium channel subunit alpha Nav1.8, Peripheral nerve sodiumchannel 3
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Purification
- Protein G Chromatography.
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Immunogène
- Fusion protein amino acids 1724-1956 (cytoplamic C-terminus) of Rat SCN10A/NAV1. 8
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Isotype
- IgG2a
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anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibody (Biotin)
SCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 Biotin
anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibody (FITC)SCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 FITC
anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibody (PerCP)SCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 PerCP
anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibody (PE)SCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 PE
anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibody (Atto 390)SCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 Atto 390
anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibody (Atto 488)SCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 Atto 488
anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibody (Atto 594)SCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 Atto 594
anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibody (HRP)SCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 HRP
anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibody (APC)SCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 APC
anti-Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A) (AA 1724-1956) antibodySCN10A Reactivité: Rat IHC, WB, IF, ICC, AA Hôte: Souris Monoclonal S134 unconjugated
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Indications d'application
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Western blot: 1 μg/mL (if results are poor, use the lysate in SDS buffer but without boiling,incubate for 20 mins at 37 °C).1 μg/mL was sufficient for detection of Nav1.8in 10 μg COS cell lysate transiently expressingNav1.8 by colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as thesecondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
Date de péremption
- 12 months
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- Nav1.8 (SCN10A) (Sodium Channel, Voltage-Gated, Type X, alpha Subunit (SCN10A))
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Autre désignation
- SCN10A / PN3
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Sujet
- Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). NAV1.8 is a voltage-gated sodium channel and plays a critical role in the generation and conduction of action potentials and is thus important for electrical signaling by most excitable cells. Therapeutically, the association of pain insensitivity with the loss of function of a certain sodium channel may have implications. Since Nav1.8 is not present in cardiac muscle or neurons in the central nervous system, blockers of Nav1.8 will not have direct action on these cells and thus can have less side effects than current pain medications. By performing more studies, there is a possibility to develop a new generation of drugs that can reduce the pain intensity in animals (3).Synonyms: Peripheral nerve sodium channel 3, Sodium channel protein type 10 subunit alpha, Sodium channel protein type X subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.8
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ID gène
- 29571
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UniProt
- Q62968
Antigène
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