GNB2L1 anticorps (AA 113-317)
(5 références)Aperçu rapide pour GNB2L1 anticorps (AA 113-317) (ABIN967797)
Antigène
Voir toutes GNB2L1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
Clone
-
-
Épitope
- AA 113-317
-
Réactivité croisée
- Humain, Boeuf (Vache), Poulet, Chien, Grenouille, Souris
-
Attributs du produit
-
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
6. Triton is a trademark of the Dow Chemical Company. -
Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
-
Immunogène
- Rat RACK1 aa 113-317
-
Isotype
- IgM
-
-
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (AA 2-317) antibody
GNB2L1 Reactivité: Humain, Souris, Rat WB, FACS, IF, ICC Hôte: Lapin Polyclonal unconjugated
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) antibodyKD Validated GNB2L1 Reactivité: Humain WB, FACS Hôte: Lapin Monoclonal 23GB2355 unconjugated Recombinant Antibody
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (Internal Region) antibodyVerified GNB2L1 Reactivité: Humain, Souris, Rat WB, IHC, ELISA Hôte: Chèvre Polyclonal unconjugated
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (C-Term) antibodyGNB2L1 Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye, Lapin, Mouton, Poisson zèbre (Danio rerio), Poulet, Xenopus laevis, Porc, Roussette (Chauve-souris), Singe WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (C-Term) antibodyGNB2L1 Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye, Lapin, Mouton, Poisson zèbre (Danio rerio), Saccharomyces cerevisiae, Cheval WB, IHC Hôte: Lapin Polyclonal unconjugated
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (AA 93-317) antibodyGNB2L1 Reactivité: Humain IHC, ELISA, IF Hôte: Lapin Polyclonal unconjugated
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) antibodyGNB2L1 Reactivité: Humain WB Hôte: Lapin Monoclonal unconjugated
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (AA 1-50) antibodyGNB2L1 Reactivité: Humain, Souris WB, IP Hôte: Lapin Polyclonal unconjugated
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (AA 29-58), (N-Term) antibodyGNB2L1 Reactivité: Humain WB, IHC (p) Hôte: Lapin Polyclonal RB3308 unconjugated
anti-Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1) (AA 113-317) antibodyGNB2L1 Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Poulet, Grenouille WB, IHC, IP, BI Hôte: Souris Monoclonal 20-RACK1 unconjugated
-
-
Indications d'application
-
Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument. -
Commentaires
-
Related Products: ABIN968537
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 250 μg/mL
-
Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
-
Agent conservateur
- Sodium azide
-
Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Stock
- -20 °C
-
Stockage commentaire
- Store undiluted at -20°C.
-
-
-
: "Interaction of "readthrough" acetylcholinesterase with RACK1 and PKCbeta II correlates with intensified fear-induced conflict behavior." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 1, pp. 283-8, (2003) (PubMed).
: "Protein kinase C epsilon-dependent regulation of cystic fibrosis transmembrane regulator involves binding to a receptor for activated C kinase (RACK1) and RACK1 binding to Na+/H+ exchange regulatory factor." dans: The Journal of biological chemistry, Vol. 277, Issue 25, pp. 22925-33, (2002) (PubMed).
: "The interaction of Src and RACK1 is enhanced by activation of protein kinase C and tyrosine phosphorylation of RACK1." dans: The Journal of biological chemistry, Vol. 276, Issue 23, pp. 20346-56, (2001) (PubMed).
: "Hormonal regulation of caveolae internalization." dans: The Journal of cell biology, Vol. 131, Issue 4, pp. 929-38, (1996) (PubMed).
: "Cloning of an intracellular receptor for protein kinase C: a homolog of the beta subunit of G proteins." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 3, pp. 839-43, (1994) (PubMed).
-
-
- GNB2L1 (Guanine Nucleotide Binding Protein (G Protein), beta Polypeptide 2-Like 1 (GNB2L1))
-
Autre désignation
- RACK1
-
Sujet
- Several proteins which specifically bind to PKC have been classified as RACKs (receptors for activated C-kinase). RACK1 was cloned from a rat brain cDNA expression library by screening for proteins that bind PKC in the presence of phosphatidylserine, diacylglycerol, and calcium in a PKC overlay assay. By sequence homology, RACK1 appears to belong to a superfamily that includes the ß subunit of G proteins. All of these proteins contain five to eight internal repeat elements known as WD40 motifs, which appear to have a role in protein-protein interactions. In addition, RACK1 contains two short sequences homologous to a PKC-binding sequence identified in Annexin I and in the brain PKC inhibitor KCIP. The binding of RACK1 to PKC is dose-dependent and occurs at a site on PKC that is distinct from the catalytic domain, indicating that RACK1 is not a PKC substrate.
-
Poids moléculaire
- 36 kDa
-
Pathways
- cAMP Metabolic Process, Positive Regulation of Endopeptidase Activity
Antigène
-
