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ATP1B1 anticorps (Subunit beta)

Cet anticorps Lapin Polyclonal détecte spécifiquement ATP1B1 dans WB, IF, BN PAGE et ExM. Il présente une réactivité avec des échantillons de Rat, Arabidopsis thaliana, Boeuf (Vache), Porc, Orge, Phaeodactylum tricornutum, Spinach, Seal, Pea, Plasmodium berghei, Poplar (Populus), Red Algae (Cyanidioschyzon), Robinia pseudoacacia, Salmon, Scaphoideus titanus, Selaginella martensii, Setaria viridis, Soybean, Tetrabaena socialis, Tomato, Toxoplasma gondii, Zea mays, Oryza sativa, Bacillus cereus, Barnyard Grass (Echinochloa crus-galli), Bryopsis corticulans, Camellia sinensis, Poulet, Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Coton, Dionaea muscipula, Galdieria sulphuraria, Ostreococcus tauri, Nicotiana tabacum, Nicotiana benthamiana, Neochloris oleoabundans, Nannochloropsis salina, Moniliophthora perniciosa, Migratory locust (Locusta migratoria), Helicobacter pylori et E. coli. Il a été cité dans 4+ publications.

2 µg of total protein extracted with PEB from leaf tissue of (1) Arabidopsis thaliana, (2) Spinacia oleracea, (3) Lycopersicon esculentum, (4) Glycine max, (5) Populus sp. (ATP1B1 anticorps (Subunit beta))

2 µg of total protein from (1) cow, (2) chicken, (3) pig, (4) rat, (5) salmon, (6) seal, (8) Arabidopsis thaliana, (9) Zea mays extracted with Protein Extration Buffer, PEB and separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. (ATP1B1 anticorps (Subunit beta))

2 images

(4 références)

N° du produit ABIN334537

252,31 €

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Aperçu rapide pour ATP1B1 anticorps (Subunit beta) (ABIN334537)

Antigène

Voir toutes ATP1B1 Anticorps

ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Reactivité

Rat, Arabidopsis thaliana, Boeuf (Vache), Porc, Orge, Phaeodactylum tricornutum, Spinach, Seal, Pea, Plasmodium berghei, Poplar (Populus), Red Algae (Cyanidioschyzon), Robinia pseudoacacia, Salmon, Scaphoideus titanus, Selaginella martensii, Setaria viridis, Soybean, Tetrabaena socialis, Tomato, Toxoplasma gondii, Zea mays, Oryza sativa, Bacillus cereus, Barnyard Grass (Echinochloa crus-galli), Bryopsis corticulans, Camellia sinensis, Poulet, Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Coton, Dionaea muscipula, Galdieria sulphuraria, Ostreococcus tauri, Nicotiana tabacum, Nicotiana benthamiana, Neochloris oleoabundans, Nannochloropsis salina, Moniliophthora perniciosa, Migratory locust (Locusta migratoria), Helicobacter pylori, E. coli

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp ATP1B1 est non-conjugé

Application

Western Blotting (WB), Immunofluorescence (IF), Blue-native PAGE (BN PAGE), Expansion Microscopy (ExM)

Discover our top product ATP1B1 Anticorps primaire

Épitope
8

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3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
Subunit beta

Fonction

Anti-AtpB | Beta subunit of ATP synthase (chloroplastic + mitochondrial) (rabbit antibodies)

Specificité

The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 ATP synthase and most known bacterial F-type ATP synthases. Peptide used for antibody production is located ina beta sheet, which is partly exposed near the surface of the AtpB protein.

Réactivité croisée (Details)

Arabidopsis thaliana, Bacillus cereus, Bryopsis corticulans, Camelina sinensis, Chlamydomonas reinhardtii, Chlorella vulgaris, Chromochloris zofingiensis, Cyanidioschyzon merolae, Dionaea muscipula, Echinochloa crus/galli, Escherichia coli, Gossypium hirsutum, Helicobacter pylori, Hordeum vulgare, Gladieria sulphuraria, Glycine max, Lycopersicum esulentum, Moniliophthora perniciosa, Nannochloropsis salina, Neochloris oleoabundans (chlorophyta), Nicotiana bentamiana, Nicotiana tabacum, Oryza sp. (roots, leafs, pollen), Ostreococcus tauri, Pheodactylum tricornutum CCAP 1055/1, Pisum sativum, Plasmodium berghei, Populus sp., Robinia pseudoacacia, Scaphoideus titanus, Selaginella martensii, Setaria viridis, Solanum lycopersicum, Spinacia oleracea, Tetrabaena socialis, Toxoplasma gondii, Zea maysAnimal tissues from: cow, chicken, pig, rat, salmon, seal, Locusta migratoria
Not reactive in: Archeal V-type ATP synthase

Homologie

Acinetobacter baumannii,Algae,Bacillus subtilis,Brassica napus,Cannabis sativa,Clostridioides difficile,Cyanobacteria,E.coli K-12,Eragrostis tef,Galdieria sulphuraria,Heliomicrobium modesticaldum Ice1,Manihot esculenta,Nicotiana plumbaginifolia,Saccharomyces cerevisiae,Salmonella typhimurium,Trichodesmium erythraeum,Triticum aestivum,Vitis vinifera,Zosteria marina,Yrsinia sp.

Purification

Serum

Immunogène

KLH-conjugated synthetic peptide derived from available plant
anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (AA 55-271) antibody
ATP1B1 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, ICC, IF, FACS Hôte: Lapin Polyclonal unconjugated

anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (AA 64-241) antibody
ATP1B1 Reactivité: Humain WB, IHC, ELISA, IF Hôte: Lapin Polyclonal unconjugated

anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (AA 63-303) antibody
ATP1B1 Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (Middle Region) antibody
ATP1B1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Lapin, Cobaye, Mouton, Cheval, Porc WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (AA 277-291), (Extracellular) antibody
ATP1B1 Reactivité: Souris WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (Internal Region) antibody
Verified ATP1B1 Reactivité: Humain, Souris, Rat WB, IHC, ELISA Hôte: Chèvre Polyclonal unconjugated

anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (AA 112-138) antibody
ATP1B1 Reactivité: Humain WB, IHC (p) Hôte: Lapin Polyclonal RB22310 unconjugated

anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (AA 201-250) antibody
ATP1B1 Reactivité: Humain, Souris, Rat, Cobaye, Roussette (Chauve-souris) WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (Internal Region) antibody
ATP1B1 Reactivité: Humain, Souris, Rat WB, IHC, ELISA, ICC, IF Hôte: Lapin Polyclonal unconjugated

anti-ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1) (AA 53-304) antibody
ATP1B1 Reactivité: Souris WB, IHC, ICC, IP Hôte: Lapin Polyclonal unconjugated

Indications d'application

1 : 100 (IF), 1 : 5000 (BN-PAGE), 1: 2500 (U-ExM), 1 : 2000-1 : 5 000 (WB)

Commentaires

Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.Antibody is recognizing mitochondrial form of AtpB Subota el. al (2011).This antibody can be used as a loading control for bacteria, Bacillus cereus.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

For reconstitution add 50 μL of sterile water

Buffer

Serum

Conseil sur la manipulation

Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Stock

-20 °C

Stockage commentaire

Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." dans: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." dans: Journal of plant physiology, (2011) (PubMed).

Berto, DAdamo, Bergantino, Vallese, Giacometti, Costantini: "The cyanobacterium Synechocystis sp. PCC 6803 is able to express an active [FeFe]-hydrogenase without additional maturation proteins." dans: Biochemical and biophysical research communications, (2011) (PubMed).

Ahsan, Nakamura, Komatsu: "Differential responses of microsomal proteins and metabolites in two contrasting cadmium (Cd)-accumulating soybean cultivars under Cd stress." dans: Amino acids, (2010) (PubMed).
Antigène

ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Autre désignation

AtpB

Sujet

Background: ATP synthase is the universal enzyme that synthesizes ATP from ADP and phosphate using the energy stored in a transmembrane ion gradient.
Additional information: The anti-AtpB antibody will detect the mitochondrial form of the F1 ATP synthase subcomplex, as well as the chloroplastic CF1 Atp Synthase, and most known bacterial F-type Atp Synthases. Peptide used for antibody production is located in a beta sheet, which is partly exposed near the surface of the AtpB protein.Anti-AtpB antibody was used as a loading control in Chlamydomonas reinhardtii and Synechocystis sp. PCC6803.This product can be sold containing proclin if requested

Poids moléculaire

53.9 kDa (Arabidopsis thaliana), 51.7 kDa (Synechocystis PCC 6803), 53.7 kDa (Spinacia oleracea)

ID gène

830768

UniProt

P19366, P83483, P06541, A8IQU3

Pathways

Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome