eIF4EBP2 anticorps (AA 99-120)
Aperçu rapide pour eIF4EBP2 anticorps (AA 99-120) (ABIN615304)
Antigène
Voir toutes eIF4EBP2 (EIF4EBP2) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 99-120
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Specificité
- Detects 17 and 20 kDa proteins, corresponding to the apparent molecular mass of PHAS-II and its phosphorylated state on SDS-PAGE immunoblots.
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Réactivité croisée (Details)
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Species reactivity (expected):Bovine, Canine, Porcine, Mouse and Rat.
Species reactivity (tested):Human. -
Purification
- Immunoaffinity Chromatography
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Immunogène
- EIF4EBP2 antibody was raised against human PHAS-II synthetic peptide (residues 99-120) was synthesized and the peptide coupled to KLH. .Remarks: This sequence is 96% homologous to mouse PHAS-11 over these residues
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anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2) (AA 1-120) antibody
EIF4EBP2 Reactivité: Humain ELISA, IHC, WB Hôte: Lapin Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2) antibodyEIF4EBP2 Reactivité: Humain, Souris ELISA, IHC Hôte: Lapin Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2) (AA 87-115), (C-Term) antibodyEIF4EBP2 Reactivité: Humain WB Hôte: Lapin Polyclonal RB41859 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2) (N-Term) antibodyEIF4EBP2 Reactivité: Humain, Souris, Boeuf (Vache), Chien, Porc, Rat, Chévre, Lapin, Poisson zèbre (Danio rerio), Cobaye WB Hôte: Lapin Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2) (AA 61-120) antibodyEIF4EBP2 Reactivité: Humain ELISA, WB Hôte: Souris Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2) (AA 7-56) antibodyEIF4EBP2 Reactivité: Humain, Souris, Boeuf (Vache), Chien, Porc, Rat, Chévre, Lapin, Poisson zèbre (Danio rerio), Singe WB Hôte: Lapin Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2) (AA 87-115) antibodyEIF4EBP2 Reactivité: Humain IHC, WB, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2) (AA 61-120) antibodyEIF4EBP2 Reactivité: Humain ELISA, WB Hôte: Souris Monoclonal 2G8 unconjugated
anti-Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2) (AA 1-120) antibodyEIF4EBP2 Reactivité: Humain ELISA, IHC Hôte: Lapin Polyclonal unconjugated
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Concentration
- 1,0 mg/mL
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Buffer
- BBS, pH 8.4 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
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Conseil sur la manipulation
- Avoid repeated freezing and thawing.
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Stock
- 4 °C/-20 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- eIF4EBP2 (EIF4EBP2) (Eukaryotic Translation Initiation Factor 4E Binding Protein 2 (EIF4EBP2))
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Autre désignation
- 4E-BP2
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Sujet
- PHAS-II, also known as eIF4E-binding protein II (eIF4E-BPII), is a member of a family of proteins which regulate initiation. PHAS-I and -II were found to have overlapping but different patterns of expression in tissues. PHAS-II shows the highest expression in liver and kidney where very little PHAS-I is found. The PHAS proteins regulate translation initiation by binding to the inhibitory protein eIF- 4E and blocking translation by preventing access of eIF-4G to the 5' cap of the mRNA. Both PHAS proteins are phosphorylated in response to insulin or growth factors such as EGF, PDGF and IGF-1. Phosphorylation in the appropriate site(s) promotes dissociation of PHAS/eIF-4E complexes which allows eIF-4E to bind eIF-4G(p220), thereby increasing the amount of eIF-4F complex and the rate of translation initiation. Regulation of the two protein differ because PHAS-II, unlike PHAS-I is readily phosphorylated by PKA in vitro. However increasing cAMP in cells promotes dephosphorylation of both PHAS-I and PHAS-II. Pharmacological and genetic evidence indicates that the mTOR/p70S6K pathway is involved in the control of PHAS-I and -II suggesting that these proteins may be mediators of the effects of this pathway on protein synthesis and cell proliferation.Synonyms: EIF4EBP2, Eukaryotic translation initiation factor 4E-binding protein 2, eIF4E-binding protein 2
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ID gène
- 1979
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NCBI Accession
- NP_004087
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UniProt
- Q13542
Antigène
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