AFT1 anticorps
Aperçu rapide pour AFT1 anticorps (ABIN272179)
Antigène
Voir toutes AFT1 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Specificité
- This antibody detects endogenous levels of ATF1 protein. (region surrounding Thr209)
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Réactivité croisée (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
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Pureté
- > 95 % pure by SDS-PAGE
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anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibody
AFT1 Reactivité: Humain WB Hôte: Lapin Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 Reactivité: Humain WB, ELISA, IHC (p), RNAi Hôte: Souris Monoclonal 3E7 unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 3-245) antibodyAFT1 Reactivité: Souris WB, IHC, IP, ICC Hôte: Lapin Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 Reactivité: Humain WB, IHC, FACS Hôte: Lapin Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 Reactivité: Humain WB, IF Hôte: Souris Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-271) antibodyAFT1 Reactivité: Humain WB, IHC (p), FACS Hôte: Lapin Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (C-Term) antibodyAFT1 Reactivité: Humain, Souris, Rat WB, ELISA, IHC, IF Hôte: Lapin Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 3-213) antibodyAFT1 Reactivité: Humain WB, ELISA, ICC, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (N-Term) antibodyAFT1 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Lapin, Cobaye, Singe, Hamster WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-Activating Transcription Factor 1 (AFT1) (AA 1-272) antibodyAFT1 Reactivité: Humain WB, ELISA, IHC, IHC (p) Hôte: Souris Monoclonal 3E7 unconjugated
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Indications d'application
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ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1,0 mg/mL
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Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- DO NOT FREEZE!
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Stock
- 4 °C
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Stockage commentaire
- Store the antibody undiluted at 2-8 °C.
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- AFT1 (Activating Transcription Factor 1 (AFT1))
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Autre désignation
- ATF1
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Sujet
- Eukaryotic gene transcription is regulated by sequence-specific transcription factors that bind modular cis acting promoter and enhancer elements. The ATF/ CREB transcription factor family binds the palindromic cAMP response element (CRE) octanucleotide TGACGTCA. The ATF/CREB family includes CREB-1, CREB-2 (also designated ATF-4), ATF-1, ATF-2 and ATF-3. This family of proteins contain highly divergent N-terminal domains, but share a C-terminal leucine zipper for dimerization and DNA binding. ATF-1 is shown to play a key role in the induction of NOX1, this protein binds the cAMP response element (CRE) and mediates PKA-induced stimulation of CRE-reporter genes. ATF-2 forms homodimers and heterodimers with c-Jun to initiate CREdependent transcription. Phosphorylation of ATF-2 at Thr 69 and Thr 71 by stress-activated kinases is necessary for transcriptional activation. Myc also induces phosphorylation of ATF-2 at Thr 69 and Thr 71 to prolong the half-life of ATF-2. ATF-2 functions as a histone acetyltransferase (HAT) and acetylates Histones H2B and H4 specifically in vitro.Synonyms: ATF-1, Activating transcription factor 1, Cyclic AMP-dependent transcription factor ATF-1, Protein TREB36, cAMP-dependent transcription factor ATF-1
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Poids moléculaire
- approx. 29, 36 kDa
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ID gène
- 466
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NCBI Accession
- NP_005162
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UniProt
- P18846
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Pathways
- Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Toll-Like Receptors Cascades
Antigène
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