Pour une meilleure utilisation, nous vous conseillons d’activer Javascript dans votre navigateur.

AKT 1/2/3 anticorps (pTyr312, pTyr315, pTyr316)

Cet anticorps anti-AKT 1/2/3 Polyclonal Lapin (ABIN362433) détecte spécifiquement AKT 1/2/3 dans IHC et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

N° du produit ABIN362433

268,40 €

Plus frais de livraison 40,00 € et TVA

Destination: France

Envoi sous 10 à 14 jours ouvrables

Aperçu rapide pour AKT 1/2/3 anticorps (pTyr312, pTyr315, pTyr316) (ABIN362433)

Antigène

AKT 1/2/3

Reactivité

Humain, Souris, Rat

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp AKT 1/2/3 est non-conjugé

Application

Immunohistochemistry (IHC), Immunofluorescence (IF)

Épitope
22

21

20

20

19

19

17

16

16

16

7

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr312, pTyr315, pTyr316

Specificité

The antibody detects endogenous level of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogène

Peptide sequence around phosphorylation site of pTyr315, 316, 312 (P-E-Y (p) -L-A) derived from Human AKT1, AKT2, AKT3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype

IgG
anti-AKT 1/2/3 (AA 401-479) antibody
Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-AKT 1/2/3 (pSer473) antibody
Reactivité: Humain, Souris, Rat, Boeuf (Vache), Lapin, Poulet, Poisson WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-AKT 1/2/3 (pSer472) antibody
Reactivité: Humain WB, IHC Hôte: Lapin Polyclonal unconjugated

anti-AKT 1/2/3 antibody
Reactivité: Humain, Souris, Rat, Chimpanzé WB, IHC, FACS, ELISA Hôte: Souris Monoclonal 14E5-16C8-25F6 unconjugated

anti-AKT 1/2/3 (pTyr474) antibody
Reactivité: Humain, Souris WB, FACS, IF (cc), IF (p), ELISA, IHC (p), ICC, IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody
Reactivité: Humain, Souris, Rat WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibody
Reactivité: Humain, Souris, Rat WB, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-AKT 1/2/3 (AA 278-312) antibody
Reactivité: Humain, Rat WB, IHC (p) Hôte: Lapin Polyclonal RB43841 unconjugated

anti-AKT 1/2/3 (pThr305), (pThr308), (pThr309) antibody
Reactivité: Humain, Souris, Rat WB, FACS, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) Hôte: Lapin Polyclonal unconjugated

anti-AKT 1/2/3 (AA 431-480) antibody
Reactivité: Humain, Souris, Rat WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated

Indications d'application

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C/-20 °C

Stockage commentaire

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Antigène

AKT 1/2/3

Autre désignation

AKT1/AKT2/AKT3

Sujet

General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.

Poids moléculaire

60 kDa

NCBI Accession

NP_005456, NP_001617

UniProt

Q9Y243