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MAP1LC3A anticorps (N-Term)

Cité dans 24 publications. Cet anticorps anti-MAP1LC3A Polyclonal Lapin (Clone RB7609) (ABIN388464) détecte spécifiquement MAP1LC3A dans WB, IF et IHC (p). L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Formalin-fixed and paraffin-embedded human brain tissue reacted with Autophagy LC3 Antibody (G8a) (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (MAP1LC3A anticorps (N-Term))

IHC (p)

Fluorescent image of cells stained with (ABIN388464 and ABIN2849519) LC3 (G8A) (N-term) antibody. (MAP1LC3A anticorps (N-Term))

3 images

(24 références)

N° du produit ABIN388464

434,77 €

Plus frais de livraison 40,00 € et TVA

Destination: France

Envoi sous 14 à 17 jours ouvrables

Aperçu rapide pour MAP1LC3A anticorps (N-Term) (ABIN388464)

Antigène

Voir toutes MAP1LC3A Anticorps

MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Reactivité

Humain, Souris, Rat

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp MAP1LC3A est non-conjugé

Application

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RB7609

Discover our top product MAP1LC3A Anticorps primaire

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AA 1-30, N-Term

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène

This LC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1~30 amino acids from the N-term of human LC3 (APG8a).

Isotype

Ig Fraction
anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 89-120), (cleaved) antibody
MAP1LC3A Reactivité: Humain, Souris WB, IF, ICC, IHC (p) Hôte: Lapin Polyclonal RB38908 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term) antibody
MAP1LC3A Reactivité: Humain, Souris, Rat WB, IF, IHC (p) Hôte: Lapin Polyclonal RB7481 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-121) antibody
MAP1LC3A Reactivité: Humain IHC, ELISA, FACS Hôte: Souris Monoclonal 5B10D8 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 77-106), (C-Term) antibody
MAP1LC3A Reactivité: Humain WB, IHC (p) Hôte: Lapin Polyclonal RB7485 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-121) antibody
MAP1LC3A Reactivité: Humain WB, IHC, ELISA, FACS Hôte: Souris Monoclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-30), (N-Term), (pSer12) antibody
MAP1LC3A Reactivité: Humain WB, DB, EIA Hôte: Lapin Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibody
MAP1LC3A Reactivité: Humain, Souris, Rat WB, IF, IHC (p), EIA Hôte: Souris Monoclonal 166AT1234 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 1-120) antibody
MAP1LC3A Reactivité: Humain, Souris, Rat WB, IHC, ELISA Hôte: Lapin Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) (AA 30-56) antibody
MAP1LC3A Reactivité: Humain WB, IF, IHC (p) Hôte: Lapin Polyclonal RB11840 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A) antibody
MAP1LC3A Reactivité: Humain WB, IHC, IP Hôte: Lapin Monoclonal unconjugated

Indications d'application

IF: 1:200. WB: 1000. IHC-P: 1:50~100

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption

6 months
Doucet-Beaupré, Gilbert, Profes, Chabrat, Pacelli, Giguère, Rioux, Charest, Deng, Laguna, Ericson, Perlmann, Ang, Cicchetti, Parent, Trudeau, Lévesque: "Lmx1a and Lmx1b regulate mitochondrial functions and survival of adult midbrain dopaminergic neurons." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 30, pp. E4387-96, (2018) (PubMed).

Lord, Farrugia, Yan, Vassie, Whitelock: "Hyaluronan coated cerium oxide nanoparticles modulate CD44 and reactive oxygen species expression in human fibroblasts." dans: Journal of biomedical materials research. Part A, Vol. 104, Issue 7, pp. 1736-46, (2017) (PubMed).

Sumiyoshi, Ishitobi, Miyaki, Miyado, Adachi, Ochi: "The role of tetraspanin CD9 in osteoarthritis using three different mouse models." dans: Biomedical research (Tokyo, Japan), Vol. 37, Issue 5, pp. 283-291, (2017) (PubMed).

Paquet, Nicoll, Love, Mouton-Liger, Holmes, Hugon, Boche: "Downregulated apoptosis and autophagy after anti-Aβ immunotherapy in Alzheimer's disease." dans: Brain pathology (Zurich, Switzerland), (2017) (PubMed).

Chen, Akinyemi, Hase, Firbank, Ndungu, Foster, Craggs, Washida, Okamoto, Thomas, Polvikoski, Allan, Oakley, OBrien, Horsburgh, Ihara, Kalaria: "Frontal white matter hyperintensities, clasmatodendrosis and gliovascular abnormalities in ageing and post-stroke dementia." dans: Brain : a journal of neurology, Vol. 139, Issue Pt 1, pp. 242-58, (2016) (PubMed).

So, Chen, Wang, Wu, Huang, Lai, Pan, Kang, Huang: "Midazolam regulated caspase pathway, endoplasmic reticulum stress, autophagy, and cell cycle to induce apoptosis in MA-10 mouse Leydig tumor cells." dans: OncoTargets and therapy, Vol. 9, pp. 2519-33, (2016) (PubMed).

Castro, Gao, Moore, Yu, Di, Kissling, Dixon: "A High Concentration of Genistein Induces Cell Death in Human Uterine Leiomyoma Cells by Autophagy." dans: Expert opinion on environmental biology, Vol. 5, Issue Suppl 1, (2016) (PubMed).

Takada, Miyaki, Ishitobi, Hirai, Nakasa, Igarashi, Lotz, Ochi: "Bach1 deficiency reduces severity of osteoarthritis through upregulation of heme oxygenase-1." dans: Arthritis research & therapy, Vol. 17, pp. 285, (2015) (PubMed).

Zhu, Tong, Teh, Wang: "Forkhead box protein O3 transcription factor negatively regulates autophagy in human cancer cells by inhibiting forkhead box protein O1 expression and cytosolic accumulation." dans: PLoS ONE, Vol. 9, Issue 12, pp. e115087, (2014) (PubMed).

Zhang, Yi, Ko, Kim, Seo, Lee, Lee, Kim: "Expression of LC3 and Beclin 1 in the spinal dorsal horn following spinal nerve ligation-induced neuropathic pain." dans: Brain research, Vol. 1519, pp. 31-9, (2013) (PubMed).

Nascimento-Ferreira, Santos-Ferreira, Sousa-Ferreira, Auregan, Onofre, Alves, Dufour, Colomer Gould, Koeppen, Déglon, Pereira de Almeida: "Overexpression of the autophagic beclin-1 protein clears mutant ataxin-3 and alleviates Machado-Joseph disease." dans: Brain : a journal of neurology, Vol. 134, Issue Pt 5, pp. 1400-15, (2011) (PubMed).

Lee, Liao, Tsai, Chiu, Wen: "Identification and anti-human glioblastoma activity of tagitinin C from Tithonia diversifolia methanolic extract." dans: Journal of agricultural and food chemistry, Vol. 59, Issue 6, pp. 2347-55, (2011) (PubMed).

Bains, Zaegel, Mize-Berge, Heidenreich: "IGF-I stimulates Rab7-RILP interaction during neuronal autophagy." dans: Neuroscience letters, Vol. 488, Issue 2, pp. 112-7, (2011) (PubMed).

Cirstea, Hideshima, Rodig, Santo, Pozzi, Vallet, Ikeda, Perrone, Gorgun, Patel, Desai, Sportelli, Kapoor, Vali, Mukherjee, Munshi, Anderson, Raje: "Dual inhibition of akt/mammalian target of rapamycin pathway by nanoparticle albumin-bound-rapamycin and perifosine induces antitumor activity in multiple myeloma." dans: Molecular cancer therapeutics, Vol. 9, Issue 4, pp. 963-75, (2010) (PubMed).

Li, Castano, Hudson, Nowlin, Lin, Bonventre, Swanson, Duffield: "The melanoma-associated transmembrane glycoprotein Gpnmb controls trafficking of cellular debris for degradation and is essential for tissue repair." dans: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 12, pp. 4767-81, (2010) (PubMed).

Wang, Hossain, Tan, Coolman, Zhou, Liu, Casey: "Inhibition of isoprenylcysteine carboxylmethyltransferase induces autophagic-dependent apoptosis and impairs tumor growth." dans: Oncogene, Vol. 29, Issue 35, pp. 4959-70, (2010) (PubMed).

Hino, Hamada, Tajika, Funayama, Morimura, Sakashita, Yokota, Fukamoto, Mutou, Kobayashi, Yorifuji: "Heavy ion irradiation induces autophagy in irradiated C2C12 myoblasts and their bystander cells." dans: Journal of electron microscopy, Vol. 59, Issue 6, pp. 495-501, (2010) (PubMed).

Li, Ma, Wang, Liu, Yang, Zhao, Wang, Jin, Zeng, Li, Song, Li, Li, Qian, Cao: "c-Abl and Arg tyrosine kinases regulate lysosomal degradation of the oncoprotein Galectin-3." dans: Cell death and differentiation, Vol. 17, Issue 8, pp. 1277-87, (2010) (PubMed).

Geng, Kohli, Klocke, Roth: "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent." dans: Neuro-oncology, Vol. 12, Issue 5, pp. 473-81, (2010) (PubMed).

Bohensky, Leshinsky, Srinivas, Shapiro: "Chondrocyte autophagy is stimulated by HIF-1 dependent AMPK activation and mTOR suppression." dans: Pediatric nephrology (Berlin, Germany), Vol. 25, Issue 4, pp. 633-42, (2010) (PubMed).

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Antigène

MAP1LC3A (Microtubule-Associated Protein 1 Light Chain 3 alpha (MAP1LC3A))

Autre désignation

LC3

Sujet

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3a is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Poids moléculaire

14272

ID gène

84557

NCBI Accession

NP_115903, NP_852610

UniProt

Q9H492

Pathways

Autophagy