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LC3B anticorps (cleaved)

Il existe 11+ publications pour ce produit. L’anticorps anti-LC3B Polyclonal Lapin est utilisé pour la détection de LC3B dans des échantillons de Humain et Souris. Il a été validé pour IF et ICC.

Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. (LC3B anticorps (cleaved))

SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4 % of paraformaldehyde (right panel). (LC3B anticorps (cleaved))

ICC

Fluorescent image of cells stained with cleaved LC3B antibody. (LC3B anticorps (cleaved))

3 images

(11 références)

N° du produit ABIN388484

434,77 €

Plus frais de livraison 40,00 € et TVA

Destination: France

Envoi sous 14 à 17 jours ouvrables

Aperçu rapide pour LC3B anticorps (cleaved) (ABIN388484)

Antigène

Voir toutes LC3B (MAP1LC3B) Anticorps

LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivité

Humain, Souris

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp LC3B est non-conjugé

Application

Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone

RB15839-RB28608

Discover our top product MAP1LC3B Anticorps primaire

Épitope
16

12

9

8

5

5

5

5

5

4

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 89-122, cleaved

Homologie

B

Purification

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène

This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.

Isotype

Ig Fraction
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B Reactivité: Rat WB, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B Reactivité: Humain, Souris, Rat WB, IHC (p), FACS Hôte: Lapin Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B Reactivité: Humain ELISA, FACS Hôte: Souris Monoclonal 5H12 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
KD Validated MAP1LC3B Reactivité: Humain WB, FACS, IF (p) Hôte: Lapin Monoclonal 25GB3515 unconjugated Recombinant Antibody

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-30), (N-Term) antibody
MAP1LC3B Reactivité: Humain, Rat WB, IF, IHC (p), EIA Hôte: Lapin Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 74-106) antibody
MAP1LC3B Reactivité: Humain, Souris WB, ELISA, IHC, FACS Hôte: Lapin Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibody
MAP1LC3B Reactivité: Humain, Souris, Rat WB, ELISA, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 77-106) antibody
MAP1LC3B Reactivité: Humain WB, ELISA, IHC Hôte: Lapin Polyclonal unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (pThr12) antibody
MAP1LC3B Reactivité: Humain DB Hôte: Lapin Polyclonal RB12679 unconjugated

anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-125) antibody
MAP1LC3B Reactivité: Humain WB, ELISA Hôte: Souris Monoclonal 4G7 unconjugated

Indications d'application

IF: 1:100. IF: 1:10~50. ICC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Date de péremption

6 months
Ock, Park, Han, Jeong, Kim, Lee, Hahm: "Genetic ablation or pharmacologic inhibition of autophagy mitigated NSAID-associated gastric damages." dans: Journal of molecular medicine (Berlin, Germany), Vol. 95, Issue 4, pp. 405-416, (2018) (PubMed).

Xu, Huai, Meng, Dong, Liu, Qi, Hu, Fan, Jin, Lv: "L-3-n-Butylphthalide Activates Akt/mTOR Signaling, Inhibits Neuronal Apoptosis and Autophagy and Improves Cognitive Impairment in Mice with Repeated Cerebral Ischemia-Reperfusion Injury." dans: Neurochemical research, Vol. 42, Issue 10, pp. 2968-2981, (2018) (PubMed).

Filipczak, Thomas, Chen, Salzman, McDonald, Lin, Belinsky: "TSC2 Deficiency Unmasks a Novel Necrosis Pathway That Is Suppressed by the RIP1/RIP3/MLKL Signaling Cascade." dans: Cancer research, Vol. 76, Issue 24, pp. 7130-7139, (2017) (PubMed).

Lee, Park, Hahm: "Mitigated NSAID-induced apoptotic and autophagic cell death with Smad7 overexpression." dans: Journal of clinical biochemistry and nutrition, Vol. 60, Issue 1, pp. 55-62, (2017) (PubMed).

Jutten, Keulers, Schaaf, Savelkouls, Theys, Span, Vooijs, Bussink, Rouschop: "EGFR overexpressing cells and tumors are dependent on autophagy for growth and survival." dans: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 108, Issue 3, pp. 479-83, (2013) (PubMed).

Radtke, English, Rondeau, Leib, Lippé, Desjardins: "Inhibition of the host translation shutoff response by herpes simplex virus 1 triggers nuclear envelope-derived autophagy." dans: Journal of virology, Vol. 87, Issue 7, pp. 3990-7, (2013) (PubMed).

Checinska, Soengas: "The gluttonous side of malignant melanoma: basic and clinical implications of macroautophagy." dans: Pigment cell & melanoma research, Vol. 24, Issue 6, pp. 1116-32, (2011) (PubMed).

Cherra, Kulich, Uechi, Balasubramani, Mountzouris, Day, Chu: "Regulation of the autophagy protein LC3 by phosphorylation." dans: The Journal of cell biology, Vol. 190, Issue 4, pp. 533-9, (2010) (PubMed).

Eby, Rosenbluth, Mays, Marshall, Barton, Sinha, Johnson, Tang, Pietenpol: "ISG20L1 is a p53 family target gene that modulates genotoxic stress-induced autophagy." dans: Molecular cancer, Vol. 9, pp. 95, (2010) (PubMed).

Mitroulis, Kourtzelis, Kambas, Rafail, Chrysanthopoulou, Speletas, Ritis: "Regulation of the autophagic machinery in human neutrophils." dans: European journal of immunology, Vol. 40, Issue 5, pp. 1461-72, (2010) (PubMed).

English, Chemali, Duron, Rondeau, Laplante, Gingras, Alexander, Leib, Norbury, Lippé, Desjardins: "Autophagy enhances the presentation of endogenous viral antigens on MHC class I molecules during HSV-1 infection." dans: Nature immunology, Vol. 10, Issue 5, pp. 480-7, (2009) (PubMed).
Antigène

LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Autre désignation

LC3B

Sujet

Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.

Poids moléculaire

14688

ID gène

81631

NCBI Accession

NP_073729

UniProt

Q9GZQ8

Pathways

Autophagy