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SMURF1 anticorps (N-Term)

Cet anticorps Lapin Polyclonal détecte spécifiquement SMURF1 dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain et Souris. Il a été cité dans 4+ publications.

Formalin-fixed and paraffin-embedded human Testis tissue reacted with hSMURF1-W81 (ABIN388828 and ABIN2850430) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (SMURF1 anticorps (N-Term))

IHC (p)

All lanes : Anti-SMURF1 Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 μg per lane. (SMURF1 anticorps (N-Term))

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with hSMURF1-W81 (ABIN388828 and ABIN2850430) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (SMURF1 anticorps (N-Term))

IHC (p)

4 images

(4 références)

N° du produit ABIN388828

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Aperçu rapide pour SMURF1 anticorps (N-Term) (ABIN388828)

Antigène

Voir toutes SMURF1 Anticorps

SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Reactivité

Humain, Souris

Hôte

Lapin

Clonalité

Polyclonal

Conjugué

Cet anticorp SMURF1 est non-conjugé

Application

Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone

RB04101

Discover our top product SMURF1 Anticorps primaire

Épitope
8

8

7

7

6

3

2

2

2

2

1

1

1

1
AA 66-96, N-Term

Homologie

X

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogène

This SMURF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-96 amino acids from the N-terminal region of human SMURF1.

Isotype

Ig Fraction
anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-268) antibody
SMURF1 Reactivité: Humain WB, ELISA, IF, IHC (p), IP Hôte: Souris Monoclonal 1D7 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 198-374) antibody
SMURF1 Reactivité: Humain WB, ELISA, IHC, IF Hôte: Lapin Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) antibody
SMURF1 Reactivité: Humain, Souris WB, IF, IHC (p), EIA, IP Hôte: Souris Monoclonal 1D7 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 711-740), (C-Term) antibody
SMURF1 Reactivité: Humain WB, IHC (p) Hôte: Lapin Polyclonal RB4103 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 1-731) antibody
SMURF1 Reactivité: Humain WB, PLA Hôte: Lapin Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-269) antibody
SMURF1 Reactivité: Humain, Souris WB, ELISA, IHC, IF, IHC (p), IP Hôte: Souris Monoclonal 1D7 unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 66-96) antibody
SMURF1 Reactivité: Humain, Souris WB, IHC, IHC (p) Hôte: Lapin Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (N-Term) antibody
SMURF1 Reactivité: Humain, Souris WB, IHC (p), EIA Hôte: Lapin Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-268) antibody
SMURF1 Reactivité: Humain WB, ELISA Hôte: Souris Polyclonal unconjugated

anti-SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (C-Term) antibody
SMURF1 Reactivité: Humain, Souris WB, IHC (p), EIA Hôte: Lapin Polyclonal unconjugated

Indications d'application

WB: 1:1000. WB: 1:2000. IHC-P: 1:10~50. IHC-P: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur

Sodium azide

Précaution d'utilisation

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation

Avoid freeze-thaw cycles.

Stock

4 °C,-20 °C

Stockage commentaire

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Date de péremption

6 months
He, Yang, Isales, Shi: "Glucocorticoid-induced leucine zipper (GILZ) antagonizes TNF-? inhibition of mesenchymal stem cell osteogenic differentiation." dans: PLoS ONE, Vol. 7, Issue 3, pp. e31717, (2012) (PubMed).

Guo, Yamashita, Zhang, Zhou, Chen, Reynolds, Awad, Yanoso, Zhao, Schwarz, Zhang, Boyce, Xing: "Ubiquitin ligase Smurf1 mediates tumor necrosis factor-induced systemic bone loss by promoting proteasomal degradation of bone morphogenetic signaling proteins." dans: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23084-92, (2008) (PubMed).

Schwamborn, Müller, Becker, Püschel: "Ubiquitination of the GTPase Rap1B by the ubiquitin ligase Smurf2 is required for the establishment of neuronal polarity." dans: The EMBO journal, Vol. 26, Issue 5, pp. 1410-22, (2007) (PubMed).

Kaneki, Guo, Chen, Yao, Schwarz, Zhang, Boyce, Xing: "Tumor necrosis factor promotes Runx2 degradation through up-regulation of Smurf1 and Smurf2 in osteoblasts." dans: The Journal of biological chemistry, Vol. 281, Issue 7, pp. 4326-33, (2006) (PubMed).
Antigène

SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))

Autre désignation

SMURF1

Sujet

Members of the transforming growth factor-beta (TGFB) family signal through type I and type II serine/threonine-kinase receptors, which in turn activate the SMAD signaling pathway. Bone morphogenetic protein (BMP) receptors target SMAD1, SMAD5, and SMAD8, whereas receptors for activin and TGFB (e.g., ACVR1 and TGFBR1, respectively) target SMAD2 and SMAD3. Phosphorylation of these receptor-regulated SMADs induces their association with the common-partner SMAD, SMAD4. Smurf1, a HECT domain E3 ubiquitin ligase, regulates cell polarity and protrusive activity and is required to maintain the transformed morphology and motility of a tumor cell. Atypical protein kinase C-zeta (PKC2), an effector of the Cdc42/Rac1-PAR6 polarity complex, recruits Smurf1 to cellular protrusions, where it controlled the local level of RhoA. Smurf1 thus links the polarity complex to degradation of RhoA in lamellipodia and filopodia to prevent RhoA signaling during dynamic membrane movements.

Poids moléculaire

86114

ID gène

57154

NCBI Accession

NP_001186776, NP_065162, NP_851994

UniProt

Q9HCE7

Pathways

Regulation of Cell Size