SPRY4 anticorps (AA 275-299)
Aperçu rapide pour SPRY4 anticorps (AA 275-299) (ABIN129626)
Antigène
Voir toutes SPRY4 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 275-299
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N° du produit (Fournisseur)
- 600-401-697
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Fournisseur
- Rockland
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Fonction
- Sprouty-4 Antibody
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Réactivité croisée (Details)
- This affinity purified antibody is directed against human Sprouty-4 protein.
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Attributs du produit
- Synonyms: Rabbit anti-Sprouty4 antibody, Rabbit anti-Sprouty-4 antibody, Sprouty 4, SPRY4, SPRY 4, Spry-4, Protein sprouty homolog 4
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Purification
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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Stérilité
- Sterile filtered
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Immunogène
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Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal region near amino acids 275-299 of Human Sprouty-4 protein.
Immunogen Type: Conjugated Peptide
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Isotype
- IgG
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anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 26-56) antibody
SPRY4 Reactivité: Humain WB, FACS, IHC (p) Hôte: Lapin Polyclonal RB15585 unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (Internal Region) antibodySPRY4 Reactivité: Humain, Souris, Rat WB, ELISA, IHC, IF, ICC Hôte: Lapin Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 270-301), (C-Term) antibodySPRY4 Reactivité: Humain WB Hôte: Lapin Polyclonal RB10595 unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (pTyr75) antibodySPRY4 Reactivité: Humain DB Hôte: Lapin Polyclonal RB15585 unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 1-299) antibodySPRY4 Reactivité: Humain ELISA, IF Hôte: Lapin Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (C-Term) antibodySPRY4 Reactivité: Humain WB, IHC (p) Hôte: Lapin Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (C-Term) antibodySPRY4 Reactivité: Humain, Rat, Cobaye, Cheval, Lapin, Singe, Porc WB Hôte: Lapin Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (Middle Region) antibodySPRY4 Reactivité: Humain, Souris, Rat, Boeuf (Vache), Cobaye, Cheval, Lapin WB Hôte: Lapin Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 1-297) antibodySPRY4 Reactivité: Humain WB, ELISA, IHC, FACS Hôte: Lapin Polyclonal unconjugated
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Indications d'application
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Immunohistochemistry Dilution: 1 μg/mL to 10 μg/mL
Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band (maybe a doublet) ~35 kDa in size corresponding to Sprouty-4 by western blotting in the appropriate cell lysate or extract. Some non-specific banding may be observed using this antibody.
Western Blot Dilution: 1:100 - 1:1,000
ELISA Dilution: 1:3,000 - 1:12,000
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.1 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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Date de péremption
- 12 months
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- SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
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Autre désignation
- SPRY4
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Sujet
- Background: Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
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ID gène
- 81848
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NCBI Accession
- NP_001120968
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UniProt
- Q9C004
Antigène
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