EpCAM anticorps

N° du produit ABIN111180

Détail du produit anti-EpCAM anticorps

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EpCAM est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificité: This antibody recognises the 34 kDa cell surface antigen known as CD326 or Epithelial Cell Adhesion Molecule (Ep-CAM).

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography

Immunogène: HT29 carcinoma cell line. Spleen cells from immunised BALB/c mice were fused with cells of a mouse myeloma cell line.

Clone: HEA125

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry: Use 5 μL of neat antibody to label 10^6 cells or 100 μL cell suspension. Immunohistochemistry on Frozen Sections: 1/20. Immunohistochemistry on paraffin sections: 1/20. This product requires protein digestionpre-treatment of paraffin sections e. g.0.1 % pronase, 10 minutes. Recommended Positive Control: Human colon and appendix.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.3 containing 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur EpCAM

Antigène: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Autre désignation: CD326 / EPCAM / TACSTD1 (EPCAM Produits)

Synonymes: anticorps DIAR5, anticorps EGP-2, anticorps EGP314, anticorps EGP40, anticorps ESA, anticorps HNPCC8, anticorps KS1/4, anticorps KSA, anticorps M4S1, anticorps MIC18, anticorps MK-1, anticorps TACSTD1, anticorps TROP1, anticorps ECS-1, anticorps ECS1, anticorps ESA1, anticorps M17S1, anticorps EGP, anticorps Ep-CAM, anticorps sb:cb6, anticorps tacstd, anticorps wu:fj17g02, anticorps zgc:110304, anticorps zgc:77119, anticorps tacstd1, anticorps MGC80540, anticorps EPCAM, anticorps egp, anticorps ksa, anticorps m4s1, anticorps mk-1, anticorps cd326, anticorps egp40, anticorps mic18, anticorps trop1, anticorps ep-cam, anticorps hegp-2, anticorps co17-1a, anticorps ga733-2, anticorps CD326, anticorps Egp314, anticorps EpCAM1, anticorps GA733-2, anticorps Ly74, anticorps Tacsd1, anticorps Tacstd1, anticorps gp40, anticorps epithelial cell adhesion molecule, anticorps flotillin 2, anticorps epithelial cell adhesion molecule S homeolog, anticorps EPCAM, anticorps FLOT2, anticorps epcam, anticorps epcam.S, anticorps Epcam

Sujet: This antigen has been identified independently by a number of groups, and it has been known by a variety of names including Epithelial Specific Antigen, MOC31 and Ber-EP4. Several monoclonal antibodies have been raised against Ep-CAM, many of which have been described as tumour specific molecules on carcinomas. Ep-CAM is a Type 1 transmembrane glycoprotein. It is expressed on the basolateral membrane of cells by the majority of epithelial tissues, with the exception of adult squamous epithelium and some specific epithelial cell types including hepatocytes and gastric epithelial cells. Ep-CAM expression has been reported to be a possible marker of early malignancy, with expression being increased in tumour cells, and de novo expression being seen in dysplastic squamous epithelium.Synonyms: Adenocarcinoma-associated antigen, EGP314, Ep-CAM, Epithelial cell adhesion molecule, Epithelial glycoprotein 314, GA733-2, KS 1/4 antigen, KSA, M1S2, M4S1, MIC18, Major gastrointestinal tumor-associated protein GA733-2, TROP1, Trop-1, Tumor-associated calcium signal transducer 1

ID gène: 4072

NCBI Accession: NP_002345

UniProt: P16422