Complement C4A/C4B anticorps

N° du produit ABIN111802

Détail du produit

Antigène: Complement C4A/C4B

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificité: This antibody recognises the secreted protein complement component 4d (C4d). C4d was accepted in 2003 into the Banff classification for identification of acute AMR (Racusen et al. 2003). Mouse anti Human C4d antibody, clone 10-11 is specific for C4d, a marker that can be used in the detection of acute AMR for kidney, heart, pancreas and lung allografts. C4d is regarded as a key marker of antibody-mediated cell injury and humoral rejection (Sacks and Chowdhury 2002). Complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4b's alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonization and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4b's short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation (Collins et al. 1999). Mouse anti Human C4d antibody, clone 10-11 is used to detect the biomarker C4d which has been described as a

Purification: Affinity chromatography on Protein A.

Immunogène: Native human C4 from plasma

Clone: 10-11-14

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on Frozen Sections (1/100-1/750): works very well on acetone-fixed,frozen renal biopsies, Strong staining is observed in the glomeruli and in some cases theperitubular capillaries. Immunohistochemistry on Paraffin Sections: variable result on formalin-fixed,paraffin-embedded sections, It has been observed that pre-treatment with 88 % formic acidfor 20 minutes at room temperature is beneficial. ELISA (1/5000-1/20000). Western Blot. Immunofluorescence (1/250-1/600).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Borate buffered saline, 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détail du antigène

Antigène: Complement C4A/C4B

Synonymes: anticorps C4, anticorps C4-1, anticorps C4b, anticorps complement component 4A (Rodgers blood group), anticorps C4a

Sujet: The complement 1 complex cleaves complement 4 (C4) to form C4b and C4a. C4b levels are strictly regulated. Single site cleavage of the C4bs alpha chain by Factor I forms iC4b and blocks C3 convertase, inhibiting opsonisation and activation of the classical pathway. This requires C4 binding protein or CR1 as a cofactor. iC4b is further degraded into C4d and C4c. C4ds short half life means that C4d is present in serum at high enough concentrations to make it a useful marker for classical complement activation. C4 has been linked to susceptibility to systemic lupus erythematosous.Synonyms: Acidic complement C4, Basic complement C4, CPAMD2, CPAMD3, Complement component 4

ID gène: 720

NCBI Accession: NP_009224

UniProt: P0C0L4