ATG12 anticorps

N° du produit ABIN116953

Détail du produit anti-ATG12 anticorps

Antigène: ATG12 (Autophagy Related 12 (ATG12))

Reactivité: Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ATG12 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Réactivité croisée (Details): Species reactivity (tested):S. cerevisiae, Yeast

Purification: A multi-step process which includes Delipidation, Salt Fractionation and Ion exchange chromatography followed by extensive dialysis against the buffer stated below.

Immunogène: This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast APG12 protein.

Information d'application

Indications d'application: This purified polyclonal antibody reacts with yeast APG12 by Western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or conjugates).
Other intrinsic bands are readily detectable at lower dilutions. A 22.1 kDa band corresponding to yeast APG12 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/200. Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore with 0.1 mL of deionized water or equivalent.

Concentration: 5.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Stock: 4 °C/-20 °C

Stockage commentaire: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Détails sur ATG12

Antigène: ATG12 (Autophagy Related 12 (ATG12))

Autre désignation: ATG12 / APG12 (ATG12 Produits)

Synonymes: anticorps APG12, anticorps APG12L, anticorps FBR93, anticorps HAPG12, anticorps Apg12l, anticorps AUTOPHAGY 12, anticorps AUTOPHAGY 12 B, anticorps CG10861, anticorps Dmel\\CG10861, anticorps atg12, anticorps ATG12, anticorps apg12, anticorps DDBDRAFT_0185323, anticorps DDBDRAFT_0191412, anticorps DDB_0185323, anticorps DDB_0191412, anticorps 4931423H11Rik, anticorps A330058M13Rik, anticorps Atg12l, anticorps AO090001000593, anticorps autophagy related 12, anticorps Ubiquitin-like superfamily protein, anticorps Atg12p, anticorps autophagy protein, anticorps ubiquitin-like protein ATG12B, anticorps autophagy associated protein Atg12, anticorps Autophagy-related 12, anticorps ATG12 autophagy related 12 homolog (S. cerevisiae), anticorps ATG12 autophagy related 12 homolog (S. cerevisiae) pseudogene, anticorps autophagy associated ubiquitin-like modifier Atg12, anticorps hypothetical protein, anticorps Autophagy-related protein 12 (Autophagy-related ubiquitin-like modifier ATG12), anticorps autophagy protein 12, anticorps autophagy related 12 S homeolog, anticorps autophagy-related protein 12, anticorps ATG12, anticorps Atg12, anticorps APG12B, anticorps APG12, anticorps LOC9321066, anticorps apg12, anticorps atg12, anticorps LOC743425, anticorps CAALFM_C113950CA, anticorps atg12.S, anticorps ANI_1_922094, anticorps AOR_1_1064164

Sujet: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.Synonyms: APG12-like, APG12L, Autophagy-related protein 12, Ubiquitin-like protein ATG12

ID gène: 852518

NCBI Accession: NP_009776

UniProt: P38316

Pathways: Autophagy