APP anticorps (Amyloid beta (A4) Precursor Protein) (AA 666-670)

Details for Product anti-APP Antibody No. ABIN197432, Fournisseur: Connectez-vous pour afficher
Antigène
  • APP
  • APP-like
  • APPL
  • Abeta
  • BcDNA:GH04413
  • CG7727
  • Dmel\\CG7727
  • EG:65F1.5
  • appl
  • aaa
  • abeta
  • abpp
  • ad1
  • appi
  • ctfgamma
  • cvap
  • pn2
  • AAA
  • ABETA
  • ABPP
  • AD1
  • APPI
  • CTFgamma
  • CVAP
  • PN-II
  • PN2
  • Abpp
  • Adap
  • Ag
  • Cvap
  • E030013M08Rik
  • betaApp
  • app
  • wu:fj34d10
  • wu:fk65e12
  • zgc:85740
  • beta amyloid protein precursor-like
  • amyloid beta (A4) precursor protein
  • amyloid beta (A4) precursor protein a
  • beta amyloid protein precursor
  • Appl
  • APP
  • app
  • App
  • appa
  • LOC100009546
Alternatives
anti-Humain APP anticorps pour Immunofluorescence (fixed cells)
Épitope
AA 666-670
101
95
68
37
30
25
24
19
17
16
15
15
14
14
12
10
7
7
7
7
6
6
6
5
5
5
5
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivité
Humain, Souris, Rat (Rattus)
876
364
351
40
27
21
19
18
15
13
12
10
7
7
4
4
4
3
2
2
1
Hôte
Lapin
630
230
46
7
2
Clonalité
Polyclonal
Conjugué
Cet anticorp APP est non-conjugé
49
39
39
16
14
13
13
13
13
12
12
12
12
12
12
8
8
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
623
366
273
158
149
120
83
56
42
34
18
14
10
9
8
3
2
2
1
1
1
1
Options
Fournisseur
Connectez-vous pour afficher
Supplier Product No.
Connectez-vous pour afficher

Images

'Independent Validation' signe
Numéro du lot 8715
Application validée Immunofluorescence
Contrôle positif Human brain
Contrôle négative Human liver
Conclusion Signal was detected in positive control tissue, and no signal was seen in negative control tissue.
Anticorps primaire
Contrôle isotypique
Anticorps secondaire
Contrôles
  • Positive control: human brain (specimen known to contain the target protein) from Molecular Pathology
  • Core.
  • Negative Control: human liver (specimen known to not contain the target protein or express low level)
  • from Molecular Pathology Core.
  • Primary antibody isotype control: human brain treated with primary antibody isotype control instead of
  • the primary antibody.
  • Secondary antibody only control: human brain treated with secondary antibody only (no primary
  • antibody).
Protocole
  • Sections were deparaffinized and rehydrated.
  • Sections were heated to 98°C for 20 min in citrate buffer pH 6.0 (Biogenex, HK086-9K) for antigen retrieval and cooled down for 20 min on the bench.
  • Sections were blocked in 10% NGS (normal goat serum) for 20 min at room temperature.
  • Sections were washed x 2 in 1xTBS buffer.
  • Sections were incubated with primary antibody diluted 1:100 in Antibody Diluent (Invitrogen, 003218) at 4°C overnight.
  • Sections were washed x 2 in 1xTBS buffer.
  • Sections were incubated with AlexaFluor 488 Goat anti-Rabbit IgG 1:1000 for 60 min.
  • Sections were washed x 3 in 1xTBS buffer.
  • Sections were mounted with DAPI (Invitrogen, Prolong Gold antifade reagent with DAPI) and coverslipped.
  • Sections were photographed with a Zeiss Axioskop2 microscope
Notes None
Validation Images
Immunofluorescence validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197433) Figure 1. Micrograph image of positive control (human brain FFPE tissue). APP stainin...
Immunofluorescence validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197433) Figure 2: micrograph image of negative control (human liver FFPE tissue) stained with...
Immunofluorescence validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197433) Figure 3: micrograph image of isotype control (rabbit IgG isotype control antibody on...
Immunofluorescence validation image for anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (ABIN197433) Figure 4: micrograph image of secondary antibody only control (no primary antibody on...
Immunogène peptide Sequence around amino acids 666~670 (A-V-T-P-E) derived from Human APP protein.
Specificité This antibody detects endogenous levels of total APP protein.
Purification Affinity Chromatography using epitope-specific immunogen.
Autre désignation Amyloid beta A4 Protein / APP (APP Antibody Extrait)
Sujet Amyloid beta precursor protein gene (ABPP) encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Multiple transcript variants encoding several different isoforms have been found for this gene. Isoform APP695 is the predominant form in neuronal tissue, isoform APP751 and isoform APP770 are widely expressed in nonneuronal cells. Isoform APP751 is the most abundant form in T lymphocytes. ABPP is expressed in all fetal tissues examined with the highest levels in brain, kidney, heart and spleen with weak expression observed in liver, ABPP is induced during neuronal differentiation. In the adult brain, highest expression of ABPP gene is found in the frontal lobe of the cortex and in the anterior perisylvian cortex opercular gyri, moderate expression in the cerebellar cortex, the posterior perisylvian cortex opercular gyri and the temporal associated cortex. Weak expression is found in the striate, extra striate and motor cortices. Mutations in ABPP have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy).Synonyms: ABPP, APPI, Alzheimer disease amyloid protein, Amyloid Precursor Protein, CVAP, Cerebral vascular amyloid peptide, PreA4, Protease nexin-II
ID gène 351
NCBI Accession NP_000475
UniProt P05067
Domaine de recherche Neurology, Alzheimer's Disease
Pathways Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour
Indications d'application Western Blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Agent conservateur Sodium azide
Précaution d'utilisation This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation Avoid repeated freezing and thawing.
Stock -20 °C
Stockage commentaire Store the antibody (in aliquots) at -20 °C.
Supplier Images
 image for anti-APP anticorps (Amyloid beta (A4) Precursor Protein) (AA 666-670) (ABIN197432) anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody
 image for anti-APP anticorps (Amyloid beta (A4) Precursor Protein) (AA 666-670) (ABIN197432) anti-Amyloid beta (A4) Precursor Protein (APP) (AA 666-670) antibody (Image 2)
Background publications Iijima, Ando, Takeda, Satoh, Seki, Itohara, Greengard, Kirino, Nairn, Suzuki: "Neuron-specific phosphorylation of Alzheimer's beta-amyloid precursor protein by cyclin-dependent kinase 5." dans: Journal of neurochemistry, Vol. 75, Issue 3, pp. 1085-91, 2000 (PubMed).

Eng, Lund, Campenot: "Synthesis of beta-tubulin, actin, and other proteins in axons of sympathetic neurons in compartmented cultures." dans: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 1, pp. 1-9, 1999 (PubMed).

Hung, Selkoe: "Selective ectodomain phosphorylation and regulated cleavage of beta-amyloid precursor protein." dans: The EMBO journal, Vol. 13, Issue 3, pp. 534-42, 1994 (PubMed).

Suzuki, Oishi, Marshak, Czernik, Nairn, Greengard: "Cell cycle-dependent regulation of the phosphorylation and metabolism of the Alzheimer amyloid precursor protein." dans: The EMBO journal, Vol. 13, Issue 5, pp. 1114-22, 1994 (PubMed).