PPARG anticorps (Internal Region)

N° du produit ABIN214562

L’anticorps anti-PPARG Polyclonal Lapin est utilisé pour la détection de PPARG dans des échantillons de Humain, Souris, Rat, Chien, Lapin, Cobaye, Porc, Hamster, Canard, Vison, Macaque et Écureuil. Il a été validé pour WB, IHC, ELISA et IHC (p).

Aperçu rapide pour PPARG anticorps (Internal Region) (ABIN214562)

Antigène: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Reactivité: Humain, Souris, Rat, Chien, Lapin, Cobaye, Porc, Hamster, Canard, Vison, Macaque, Écureuil

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPARG est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-PPARG anticorps

Épitope: Internal Region

Specificité: Amino acids 255 -268 of human PPAR gamma isoform 1.

Purification: Immunoaffinity purified

Immunogène: Synthetic peptide from human PPARG.

Type of Immunogen: Synthetic peptide

Information d'application

Indications d'application: Approved: ELISA (1:8000 - 1:32000), IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C,-20 °C

Stockage commentaire: 4°C or -20°C, Avoid freeze-thaw cycles.

Détails sur PPARG

Antigène: PPARG (Peroxisome Proliferator-Activated Receptor gamma (PPARG))

Autre désignation: PPARG / PPAR Gamma

Sujet: Name/Gene ID: PPARG
Subfamily: NR1 Thyroid hormone-like
Family: NHR

Synonyms: PPARG, NR1C3, PPAR gamma, PPAR gamma 2, PPARG1, PPARG2, PPARgamma, CIMT1, GLM1, PPAR-gamma

ID gène: 5468

UniProt: P37231

Pathways: Signalisation MAPK, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Endopeptidase Activity, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation