CD43 anticorps (Biotin)

N° du produit ABIN2689257

Détail du produit anti-CD43 anticorps (Biotin)

Antigène: CD43 (SPN) (Sialophorin (SPN))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD43 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The S7 antibody reacts with the 115 kDa glycoform of CD43 (Ly-48, Leukosialin) which is expressed on IL-7-responsive pro-B cells, plasma cells, peritoneal and splenic CD5+ B cells (B-1 cells), granulocytes, monocytes, macrophages, platelets, natural killer cells, thymocytes, peripheral T cytotoxic/suppressor cells, and most T helper cells, but not resting conventional peripheral B cells. CD43 expression has also been detected on pluripotent hematopoietic stem cells and myeloid, lymphoid, and NK-cell progenitors in the bone marrow. Studies of CD43-deficient mice indicate that CD43 participates in the negative regulation of T-cell activation and adhesion. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.

BD Pharmingen™ Biotin Rat Anti-Mouse CD43 - Biotin - Clone S7 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Plasmacytoma MOPC-315

Clone: S7

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD43 (Biotin) (ABIN2689257)

Woodman, Johnston, Hickey, Teoh, Reinhardt, Poon, Kubes: "The functional paradox of CD43 in leukocyte recruitment: a study using CD43-deficient mice." dans: The Journal of experimental medicine, Vol. 188, Issue 11, pp. 2181-6, (1999) (PubMed).

Stockton, Cheng, Manjunath, Ardman, von Andrian: "Negative regulation of T cell homing by CD43." dans: Immunity, Vol. 8, Issue 3, pp. 373-81, (1998) (PubMed).

Thurman, Walker, Jayaraman, Manjunath, Ardman, Green: "Regulation of in vitro and in vivo T cell activation by CD43." dans: International immunology, Vol. 10, Issue 5, pp. 691-701, (1998) (PubMed).

Rolink, ten Boekel, Melchers, Fearon, Krop, Andersson: "A subpopulation of B220+ cells in murine bone marrow does not express CD19 and contains natural killer cell progenitors." dans: The Journal of experimental medicine, Vol. 183, Issue 1, pp. 187-94, (1996) (PubMed).

Manjunath, Correa, Ardman, Ardman: "Negative regulation of T-cell adhesion and activation by CD43." dans: Nature, Vol. 377, Issue 6549, pp. 535-8, (1995) (PubMed).

Moore, Bennett, Kumar: "Transplantable NK cell progenitors in murine bone marrow." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 4, pp. 1653-63, (1995) (PubMed).

Wells, Kantor, Stall: "CD43 (S7) expression identifies peripheral B cell subsets." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 12, pp. 5503-15, (1995) (PubMed).

Jones, Federsppiel, Ellies, Williams, Burgener, Duronio, Smith, Takei, Ziltener: "Characterization of the activation-associated isoform of CD43 on murine T lymphocytes." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3426-39, (1994) (PubMed).

Moore, Huang, Terstappen, Bennett, Kumar: "Expression of CD43 on murine and human pluripotent hematopoietic stem cells." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 11, pp. 4978-87, (1994) (PubMed).

Hardy, Hayakawa: "Generation of Ly-1 B cells from developmentally distinct precursors. Enrichment by stromal-cell culture or cell sorting." dans: Annals of the New York Academy of Sciences, Vol. 651, pp. 99-111, (1992) (PubMed).

Hardy, Carmack, Shinton, Kemp, Hayakawa: "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." dans: The Journal of experimental medicine, Vol. 173, Issue 5, pp. 1213-25, (1991) (PubMed).

Gulley, Ogata, Thorson, Dailey, Kemp: "Identification of a murine pan-T cell antigen which is also expressed during the terminal phases of B cell differentiation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 11, pp. 3751-7, (1988) (PubMed).

Détails sur CD43

Antigène: CD43 (SPN) (Sialophorin (SPN))

Autre désignation: CD43 (SPN Produits)

Synonymes: anticorps CD43, anticorps GALGP, anticorps GPL115, anticorps LSN, anticorps A630014B01Rik, anticorps Cd43, anticorps Galgp, anticorps Ly-48, anticorps Ly48, anticorps Lsn, anticorps Lsn1, anticorps gpL115, anticorps sialophorin, anticorps SPN, anticorps Spn

Sujet: Synonyms: Ly-48, Leukosialin

Pathways: Regulation of Leukocyte Mediated Immunity