GRP78 anticorps (AA 550-600)
Aperçu rapide pour GRP78 anticorps (AA 550-600) (ABIN298116)
Antigène
Voir toutes GRP78 (HSPA5) AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Épitope
- AA 550-600
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Specificité
- A portion of amino acids 550-600 of human BiP was used as the immunogen.
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Purification
- Protein G purified
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Immunogène
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A portion of amino acids 550-600 of human GRP78.
Type of Immunogen: Protein fragment -
Isotype
- IgG
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Indications d'application
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Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
Commentaires
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Target Species of Antibody: Human
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- PBS, 0.05 % BSA, 0.05 % sodium azide.
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Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Conseil sur la manipulation
- avoid freeze thaw cycles.
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
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Autre désignation
- HSPA5 / GRP78 / BIP
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Sujet
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Name/Gene ID: HSPA5
Synonyms: HSPA5, BIP, Heat shock 70 kDa protein 5, MIF2, GRP-78, GRP78 -
ID gène
- 3309
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UniProt
- P11021
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Pathways
- Thyroid Hormone Synthesis, ER-Nucleus Signaling
Antigène
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