ELAVL1 anticorps (AA 1-13)

N° du produit ABIN307186

Cet anticorps Lapin Polyclonal détecte spécifiquement ELAVL1 dans WB, IHC, IP et IHC (p). Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Porc, Chien, Poulet, Mouton, Singe, Roussette (Chauve-souris), Cheval et Lapin.

Aperçu rapide pour ELAVL1 anticorps (AA 1-13) (ABIN307186)

Antigène: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Porc, Chien, Poulet, Mouton, Singe, Roussette (Chauve-souris), Cheval, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ELAVL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-ELAVL1 anticorps

Épitope: AA 1-13

Specificité: Recognizes human HuR, Mr ~36kD.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Chicken (100%) Gibbon, Platypus (92%).

Purification: Affinity purified

Immunogène: KLH-conjugated, synthetic peptide corresponding to aa1-13 (MSNGYEDHMAEDC) of human HuR. The immunizing sequence is identical in chicken, mouse, and rat. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat, Sheep, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Horse, Pig, Opossum, Chicken (100%), Gibbon, Platypus (92%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Information d'application

Indications d'application: Approved: IHC, IHC-P (5 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2.0 μg/mL. Detected HuR in RIPA lysates from 3T3/A31 cells. Immunoprecipitation: 4 μg immunoprecipitated HuR from 500 μg of 3T3/A31 RIPA lysate.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in Tris-glycine, 0.15M sodium chloride, 0.05 % sodium azide,  pH 7.4, 30 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeat freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Détails sur ELAVL1

Antigène: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Autre désignation: ELAVL1 / HUR

Sujet: Name/Gene ID: ELAVL1

Synonyms: ELAVL1, ELAV1, Hua, HUR, Hu antigen R, ELAV-like protein 1, Hu-antigen R, MelG

ID gène: 1994

UniProt: Q15717

Pathways: AMPK Signaling